1qn4: Difference between revisions

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[[Image:1qn4.gif|left|200px]]


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==Crystal structure of the T(-24) Adenovirus major late promoter TATA box variant bound to wild-type TBP (Arabidopsis thaliana TBP isoform 2). TATA element recognition by the TATA box-binding protein has been conserved throughout evolution.==
The line below this paragraph, containing "STRUCTURE_1qn4", creates the "Structure Box" on the page.
<StructureSection load='1qn4' size='340' side='right'caption='[[1qn4]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1qn4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QN4 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qn4 OCA], [https://pdbe.org/1qn4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qn4 RCSB], [https://www.ebi.ac.uk/pdbsum/1qn4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qn4 ProSAT]</span></td></tr>
{{STRUCTURE_1qn4| PDB=1qn4 |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/TBP1_ARATH TBP1_ARATH] General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qn/1qn4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qn4 ConSurf].
<div style="clear:both"></div>


'''CRYSTAL STRUCTURE OF THE T(-24) ADENOVIRUS MAJOR LATE PROMOTER TATA BOX VARIANT BOUND TO WILD-TYPE TBP (ARABIDOPSIS THALIANA TBP ISOFORM 2). TATA ELEMENT RECOGNITION BY THE TATA BOX-BINDING PROTEIN HAS BEEN CONSERVED THROUGHOUT EVOLUTION'''
==See Also==
 
*[[TATA-binding protein 3D structures|TATA-binding protein 3D structures]]
 
*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
==Overview==
__TOC__
Cocrystal structures of wild-type TATA box-binding protein (TBP) recognizing 10 naturally occurring TATA elements have been determined at 2.3-1.8 A resolution, and compared with our 1.9 A resolution structure of TBP bound to the Adenovirus major late promoter (AdMLP) TATA box (5'-TATAAAAG-3'). Minor-groove recognition by the saddle-shaped protein induces the same conformational change in each of these oligonucleotides, despite variations in promoter sequence that reduce the efficiency of transcription initiation. Three molecular mechanisms explain assembly of diverse TBP-TATA element complexes. (1) T --&gt; A and A --&gt; T transversions leave the minor-groove face unchanged, permitting formation of TBP-DNA complexes on many A/T-rich core promoter sequences. (2) Cavities in the interface between TBP and the minor-groove face of the AdMLP TATA box accommodate the exocyclic NH(2) groups of G in a TACA box and in a TATAAG box. (3) Formation of a C:G Hoogsteen basepair in a TATAAAC box eliminates steric clashes that would be produced by the Watson-Crick base pair. We conclude that the structure of the TBP-TATA box complex found at the heart of the polymerase II (pol II) transcription machinery has remained constant over the course of evolution, despite variations in TBP and its DNA targets.
</StructureSection>
 
==About this Structure==
1QN4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QN4 OCA].
 
==Reference==
TATA element recognition by the TATA box-binding protein has been conserved throughout evolution., Patikoglou GA, Kim JL, Sun L, Yang SH, Kodadek T, Burley SK, Genes Dev. 1999 Dec 15;13(24):3217-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10617571 10617571]
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Burley, S K.]]
[[Category: Burley SK]]
[[Category: Kim, J L.]]
[[Category: Kim JL]]
[[Category: Kodadek, T.]]
[[Category: Kodadek T]]
[[Category: Patikoglou, G A.]]
[[Category: Patikoglou GA]]
[[Category: Sun, L.]]
[[Category: Sun L]]
[[Category: Yang, S H.]]
[[Category: Yang S-H]]
[[Category: Tbp-tata element complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:28:19 2008''

Latest revision as of 09:05, 17 April 2024

Crystal structure of the T(-24) Adenovirus major late promoter TATA box variant bound to wild-type TBP (Arabidopsis thaliana TBP isoform 2). TATA element recognition by the TATA box-binding protein has been conserved throughout evolution.Crystal structure of the T(-24) Adenovirus major late promoter TATA box variant bound to wild-type TBP (Arabidopsis thaliana TBP isoform 2). TATA element recognition by the TATA box-binding protein has been conserved throughout evolution.

Structural highlights

1qn4 is a 6 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.86Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TBP1_ARATH General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1qn4, resolution 1.86Å

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OCA
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