1qcv: Difference between revisions

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[[Image:1qcv.jpg|left|200px]]


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==RUBREDOXIN VARIANT (PFRD-XC4) FOLDS WITHOUT IRON==
The line below this paragraph, containing "STRUCTURE_1qcv", creates the "Structure Box" on the page.
<StructureSection load='1qcv' size='340' side='right'caption='[[1qcv]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1qcv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QCV FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qcv OCA], [https://pdbe.org/1qcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qcv RCSB], [https://www.ebi.ac.uk/pdbsum/1qcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qcv ProSAT]</span></td></tr>
{{STRUCTURE_1qcv| PDB=1qcv |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/RUBR_PYRFU RUBR_PYRFU] Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qc/1qcv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qcv ConSurf].
<div style="clear:both"></div>


'''RUBREDOXIN VARIANT (PFRD-XC4) FOLDS WITHOUT IRON'''
==See Also==
 
*[[Rubredoxin 3D structures|Rubredoxin 3D structures]]
 
*[[Rubredoxin PDB structures|Rubredoxin PDB structures]]
==Overview==
__TOC__
The role of surface salt bridges in protein stabilization has been a source of controversy. Here we present the NMR structure of a hyperthermophilic rubredoxin variant (PFRD-XC4) and the thermodynamic analysis of two surface salt bridges by double mutant cycles. This analysis shows that the surface side chain to side chain salt bridge between Lys 6 and Glu 49 does not stabilize PFRD-XC4. The main chain to side chain salt bridge between the N-terminus and Glu 14 was, however, found to stabilize PFRD-XC4 by 1. 5 kcal mol(-)(1). The entropic cost of making a surface salt bridge involving the protein's backbone is reduced, since the backbone has already been immobilized upon protein folding.
</StructureSection>
 
[[Category: Large Structures]]
==About this Structure==
1QCV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCV OCA].
 
==Reference==
Contribution of surface salt bridges to protein stability., Strop P, Mayo SL, Biochemistry. 2000 Feb 15;39(6):1251-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10684603 10684603]
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
[[Category: Single protein]]
[[Category: Mayo SL]]
[[Category: Mayo, S L.]]
[[Category: Strop P]]
[[Category: Strop, P.]]
[[Category: Hyperthermophile]]
[[Category: Rubredoxin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:08:26 2008''

Latest revision as of 09:03, 17 April 2024

RUBREDOXIN VARIANT (PFRD-XC4) FOLDS WITHOUT IRONRUBREDOXIN VARIANT (PFRD-XC4) FOLDS WITHOUT IRON

Structural highlights

1qcv is a 1 chain structure with sequence from Pyrococcus furiosus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RUBR_PYRFU Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

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