1pc6: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 ==Structural Genomics, NinB==
-<StructureSection load='1pc6' size='340' side='right' caption='[[1pc6]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
+<StructureSection load='1pc6' size='340' side='right'caption='[[1pc6]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1pc6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PC6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PC6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1pc6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PC6 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pc6 OCA], [http://pdbe.org/1pc6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pc6 RCSB], [http://www.ebi.ac.uk/pdbsum/1pc6 PDBsum], [http://www.topsan.org/Proteins/MCSG/1pc6 TOPSAN]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pc6 OCA], [https://pdbe.org/1pc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pc6 RCSB], [https://www.ebi.ac.uk/pdbsum/1pc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pc6 ProSAT], [https://www.topsan.org/Proteins/MCSG/1pc6 TOPSAN]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/NINB_LAMBD NINB_LAMBD]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
    <jmolCheckbox>
-     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pc/1pc6_consurf.spt"</scriptWhenChecked>
+     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pc/1pc6_consurf.spt"</scriptWhenChecked>
      <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
      <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 16: / Line 20: @@
 </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pc6 ConSurf].
 <div style="clear:both"></div>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Genetic recombination in bacteriophage lambda relies on DNA end processing by Exo to expose 3'-tailed strands for annealing and exchange by beta protein. Phage lambda encodes an additional recombinase, Orf, which participates in the early stages of recombination by supplying a function equivalent to the Escherichia coli RecFOR complex. These host enzymes assist loading of the RecA strand exchange protein onto ssDNA coated with ssDNA-binding protein. In this study, we purified the Orf protein, analyzed its biochemical properties, and determined its crystal structure at 2.5 angstroms. The homodimeric Orf protein is arranged as a toroid with a shallow U-shaped cleft, lined with basic residues, running perpendicular to the central cavity. Orf binds DNA, favoring single-stranded over duplex and with no obvious preference for gapped, 3'-tailed, or 5'-tailed substrates. An interaction between Orf and ssDNA-binding protein was indicated by far Western analysis. The functional similarities between Orf and RecFOR are discussed in relation to the early steps of recombinational exchange and the interplay between phage and bacterial recombinases.
-Functional similarities between phage lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange.,Maxwell KL, Reed P, Zhang RG, Beasley S, Walmsley AR, Curtis FA, Joachimiak A, Edwards AM, Sharples GJ Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11260-5. Epub 2005 Aug 2. PMID:16076958<ref>PMID:16076958</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 1pc6" style="background-color:#fffaf0;"></div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Bacteriophage lambda]]
+[[Category: Escherichia virus Lambda]]
-[[Category: Beasley, S]]
+[[Category: Large Structures]]
-[[Category: Edwards, A M]]
+[[Category: Beasley S]]
-[[Category: Joachimiak, A]]
+[[Category: Edwards AM]]
-[[Category: Structural genomic]]
+[[Category: Joachimiak A]]
-[[Category: Maxwell, K L]]
+[[Category: Maxwell KL]]
-[[Category: Zhang, R]]
+[[Category: Zhang R]]
-[[Category: Mcsg]]
-[[Category: PSI, Protein structure initiative]]
-[[Category: Unknown function]]