1oar: Difference between revisions

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[[Image:1oar.jpg|left|200px]]


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==Fv IgE SPE-7 in complex with Alizarin Red==
The line below this paragraph, containing "STRUCTURE_1oar", creates the "Structure Box" on the page.
<StructureSection load='1oar' size='340' side='right'caption='[[1oar]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1oar]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OAR FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZN:ALIZARIN+RED'>AZN</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
{{STRUCTURE_1oar|  PDB=1oar  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oar OCA], [https://pdbe.org/1oar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oar RCSB], [https://www.ebi.ac.uk/pdbsum/1oar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oar ProSAT]</span></td></tr>
 
</table>
'''FV IGE SPE-7 IN COMPLEX WITH ALIZARIN RED'''
== Function ==
 
[https://www.uniprot.org/uniprot/LV1B_MOUSE LV1B_MOUSE]  
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
A single antibody was shown to adopt different binding-site conformations and thereby bind unrelated antigens. Analysis by both x-ray crystallography and pre-steady-state kinetics revealed an equilibrium between different preexisting isomers, one of which possessed a promiscuous, low-affinity binding site for aromatic ligands, including the immunizing hapten. A subsequent induced-fit isomerization led to high-affinity complexes with a deep and narrow binding site. A protein antigen identified by repertoire selection made use of an unrelated antibody isomer with a wide, shallow binding site. Conformational diversity, whereby one sequence adopts multiple structures and multiple functions, can increase the effective size of the antibody repertoire but may also lead to autoimmunity and allergy.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/1oar_consurf.spt"</scriptWhenChecked>
1OAR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAR OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Antibody multispecificity mediated by conformational diversity., James LC, Roversi P, Tawfik DS, Science. 2003 Feb 28;299(5611):1362-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12610298 12610298]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oar ConSurf].
[[Category: Rattus rattus]]
<div style="clear:both"></div>
[[Category: Single protein]]
__TOC__
[[Category: James, L C.]]
</StructureSection>
[[Category: Roversi, P.]]
[[Category: Large Structures]]
[[Category: Tawfik, D.]]
[[Category: Mus musculus]]
[[Category: Allergy]]
[[Category: James LC]]
[[Category: Antibody]]
[[Category: Roversi P]]
[[Category: Conformational diversity]]
[[Category: Tawfik D]]
[[Category: Ige]]
[[Category: Multispecificity]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:36:17 2008''

Latest revision as of 11:56, 10 April 2024

Fv IgE SPE-7 in complex with Alizarin RedFv IgE SPE-7 in complex with Alizarin Red

Structural highlights

1oar is a 8 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.23Å
Ligands:, , , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LV1B_MOUSE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1oar, resolution 2.23Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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