1oar: Difference between revisions

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[[Image:1oar.gif|left|200px]]<br />
<applet load="1oar" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1oar, resolution 2.23&Aring;" />
'''FV IGE SPE-7 IN COMPLEX WITH ALIZARIN RED'''<br />


==Overview==
==Fv IgE SPE-7 in complex with Alizarin Red==
A single antibody was shown to adopt different binding-site conformations, and thereby bind unrelated antigens. Analysis by both x-ray, crystallography and pre-steady-state kinetics revealed an equilibrium, between different preexisting isomers, one of which possessed a, promiscuous, low-affinity binding site for aromatic ligands, including the, immunizing hapten. A subsequent induced-fit isomerization led to, high-affinity complexes with a deep and narrow binding site. A protein, antigen identified by repertoire selection made use of an unrelated, antibody isomer with a wide, shallow binding site. Conformational, diversity, whereby one sequence adopts multiple structures and multiple, functions, can increase the effective size of the antibody repertoire but, may also lead to autoimmunity and allergy.
<StructureSection load='1oar' size='340' side='right'caption='[[1oar]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1oar]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OAR FirstGlance]. <br>
1OAR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with CAC, NA, CL, DMS, EDO and AZN as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OAR OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZN:ALIZARIN+RED'>AZN</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oar OCA], [https://pdbe.org/1oar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oar RCSB], [https://www.ebi.ac.uk/pdbsum/1oar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oar ProSAT]</span></td></tr>
Antibody multispecificity mediated by conformational diversity., James LC, Roversi P, Tawfik DS, Science. 2003 Feb 28;299(5611):1362-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12610298 12610298]
</table>
[[Category: Rattus rattus]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/LV1B_MOUSE LV1B_MOUSE]  
[[Category: James, L.C.]]
== Evolutionary Conservation ==
[[Category: Roversi, P.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Tawfik, D.]]
Check<jmol>
[[Category: AZN]]
  <jmolCheckbox>
[[Category: CAC]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/1oar_consurf.spt"</scriptWhenChecked>
[[Category: CL]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: DMS]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: EDO]]
  </jmolCheckbox>
[[Category: NA]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oar ConSurf].
[[Category: allergy]]
<div style="clear:both"></div>
[[Category: antibody]]
__TOC__
[[Category: conformational diversity]]
</StructureSection>
[[Category: ige]]
[[Category: Large Structures]]
[[Category: multispecificity]]
[[Category: Mus musculus]]
 
[[Category: James LC]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 14:19:32 2007''
[[Category: Roversi P]]
[[Category: Tawfik D]]

Latest revision as of 11:56, 10 April 2024

Fv IgE SPE-7 in complex with Alizarin RedFv IgE SPE-7 in complex with Alizarin Red

Structural highlights

1oar is a 8 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.23Å
Ligands:, , , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LV1B_MOUSE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1oar, resolution 2.23Å

Drag the structure with the mouse to rotate

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OCA
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