1oar: Difference between revisions

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-[[Image:1oar.gif|left|200px]]<br />
-<applet load="1oar" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1oar, resolution 2.23&Aring;" />
-'''FV IGE SPE-7 IN COMPLEX WITH ALIZARIN RED'''<br />
-==Overview==
+==Fv IgE SPE-7 in complex with Alizarin Red==
-A single antibody was shown to adopt different binding-site conformations, and thereby bind unrelated antigens. Analysis by both x-ray, crystallography and pre-steady-state kinetics revealed an equilibrium, between different preexisting isomers, one of which possessed a, promiscuous, low-affinity binding site for aromatic ligands, including the, immunizing hapten. A subsequent induced-fit isomerization led to, high-affinity complexes with a deep and narrow binding site. A protein, antigen identified by repertoire selection made use of an unrelated, antibody isomer with a wide, shallow binding site. Conformational, diversity, whereby one sequence adopts multiple structures and multiple, functions, can increase the effective size of the antibody repertoire but, may also lead to autoimmunity ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12610298 (full description)]]
+<StructureSection load='1oar' size='340' side='right'caption='[[1oar]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1oar]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OAR FirstGlance]. <br>
-OAR is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]] with CAC, NA, CL, DMS, EDO and AZN as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OAR OCA]].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZN:ALIZARIN+RED'>AZN</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oar OCA], [https://pdbe.org/1oar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oar RCSB], [https://www.ebi.ac.uk/pdbsum/1oar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oar ProSAT]</span></td></tr>
-Antibody multispecificity mediated by conformational diversity., James LC, Roversi P, Tawfik DS, Science. 2003 Feb 28;299(5611):1362-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12610298 12610298]
+</table>
-[[Category: Rattus rattus]]
+== Function ==
-[[Category: Single protein]]
+[https://www.uniprot.org/uniprot/LV1B_MOUSE LV1B_MOUSE]
-[[Category: James, L.C.]]
+== Evolutionary Conservation ==
-[[Category: Roversi, P.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Tawfik, D.]]
+Check<jmol>
-[[Category: AZN]]
+  <jmolCheckbox>
-[[Category: CAC]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/1oar_consurf.spt"</scriptWhenChecked>
-[[Category: CL]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: DMS]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: EDO]]
+  </jmolCheckbox>
-[[Category: NA]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oar ConSurf].
-[[Category: allergy]]
+<div style="clear:both"></div>
-[[Category: antibody]]
+__TOC__
-[[Category: conformational diversity]]
+</StructureSection>
-[[Category: ige]]
+[[Category: Large Structures]]
-[[Category: multispecificity]]
+[[Category: Mus musculus]]
+[[Category: James LC]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:55:03 2007''
+[[Category: Roversi P]]
+[[Category: Tawfik D]]