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[[Image:1ngx.gif|left|200px]]


{{Structure
==Chimeric Germline Fab 7g12 with jeffamine fragment bound==
|PDB= 1ngx |SIZE=350|CAPTION= <scene name='initialview01'>1ngx</scene>, resolution 1.80&Aring;
<StructureSection load='1ngx' size='340' side='right'caption='[[1ngx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=JEF:O-(O-(2-AMINOPROPYL)-O&#39;-(2-METHOXYETHYL)POLYPROPYLENE+GLYCOL+500)'>JEF</scene>
<table><tr><td colspan='2'>[[1ngx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NGX FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JEF:O-(O-(2-AMINOPROPYL)-O-(2-METHOXYETHYL)POLYPROPYLENE+GLYCOL+500)'>JEF</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ngx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngx OCA], [https://pdbe.org/1ngx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ngx RCSB], [https://www.ebi.ac.uk/pdbsum/1ngx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ngx ProSAT]</span></td></tr>
|RELATEDENTRY=[[3fct|3FCT]], [[1n7m|1N7M]], [[1ngw|1NGW]], [[1ngy|1NGY]], [[1ngz|1NGZ]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ngx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngx OCA], [http://www.ebi.ac.uk/pdbsum/1ngx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ngx RCSB]</span>
== Evolutionary Conservation ==
}}
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ng/1ngx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ngx ConSurf].
<div style="clear:both"></div>


'''Chimeric Germline Fab 7g12 with jeffamine fragment bound'''
==See Also==
 
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
The crystal structure of the Michaelis complex between the Fab fragment of ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly adopts a nonplanar conformation and reveals that the antibody catalyzes the porphyrin metallation reaction by straining/distorting the bound substrate toward the transition-state configuration. The crystal structures of the Fab fragment of the germ-line precursor antibody to 7G12 and its complex with the hapten N-methylmesoporphyrin have also been solved. A comparison of these structures with the corresponding structures of the affinity-matured antibody 7G12 reveals the molecular mechanism by which the immune system evolves binding energy to catalyze this reaction.
[[Category: Homo sapiens]]
 
[[Category: Large Structures]]
==About this Structure==
[[Category: Mus musculus]]
1NGX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGX OCA].
[[Category: Andryski SE]]
 
[[Category: Beuscher AB]]
==Reference==
[[Category: Schultz PG]]
Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12552112 12552112]
[[Category: Stevens RC]]
[[Category: ]]
[[Category: Yin J]]
[[Category: Protein complex]]
[[Category: Andryski, S E.]]
[[Category: Beuscher, A B.]]
[[Category: Schultz, P G.]]
[[Category: Stevens, R C.]]
[[Category: Yin, J.]]
[[Category: antibody]]
[[Category: antigen binding fragment (fab)]]
[[Category: immunoglobulin]]
 
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