1n8v: Difference between revisions

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-[[Image:1n8v.gif|left|200px]]
-<!--
+==Chemosensory Protein in complex with bromo-dodecanol==
-The line below this paragraph, containing "STRUCTURE_1n8v", creates the "Structure Box" on the page.
+<StructureSection load='1n8v' size='340' side='right'caption='[[1n8v]], [[Resolution|resolution]] 1.39&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1n8v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mamestra_brassicae Mamestra brassicae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N8V FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.39&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDD:BROMO-DODECANOL'>BDD</scene></td></tr>
-{{STRUCTURE_1n8v|  PDB=1n8v  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n8v OCA], [https://pdbe.org/1n8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n8v RCSB], [https://www.ebi.ac.uk/pdbsum/1n8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n8v ProSAT]</span></td></tr>
+</table>
-'''Chemosensory Protein in complex with bromo-dodecanol'''
+== Function ==
+[https://www.uniprot.org/uniprot/Q9NG96_MAMBR Q9NG96_MAMBR]
+== Evolutionary Conservation ==
-==Overview==
+[[Image:Consurf_key_small.gif|200px|right]]
-Chemosensory proteins (CSPs) have been proposed to transport hydrophobic chemicals from air to olfactory or taste receptors. They have been isolated from several sensory organs of a wide range of insect species. The x-ray structure of CSPMbraA6, a 112-aa antennal protein from the moth Mamestra brassicae (Mbra), was shown to exhibit a novel type of alpha-helical fold. We have performed a structural and binding study of CSPMbraA6 to get some insights into its possible molecular function. Tryptophan fluorescence quenching demonstrates the ability of CSPMbraA6 to bind several types of semio-chemicals or surrogate ligands with microM K(d). Its crystal structure in complex with one of these compounds, 12-bromo-dodecanol, reveals extensive conformational changes on binding, resulting in the formation of a large cavity filled by three ligand molecules. Furthermore, binding cooperativity was demonstrated for some ligands, suggesting a stepwise binding. The peculiar rearrangement of CSPMbraA6 conformation and the cooperativity phenomenon might trigger the recognition of chemicals by receptors and induce subsequent signal transduction.
+Check<jmol>
+  <jmolCheckbox>
-==About this Structure==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n8/1n8v_consurf.spt"</scriptWhenChecked>
-N8V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mamestra_brassicae Mamestra brassicae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8V OCA].
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==Reference==
+  </jmolCheckbox>
-Moth chemosensory protein exhibits drastic conformational changes and cooperativity on ligand binding., Campanacci V, Lartigue A, Hallberg BM, Jones TA, Giudici-Orticoni MT, Tegoni M, Cambillau C, Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5069-74. Epub 2003 Apr 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12697900 12697900]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n8v ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Mamestra brassicae]]
-[[Category: Single protein]]
+[[Category: Cambillau C]]
-[[Category: Cambillau, C.]]
+[[Category: Campanacci V]]
-[[Category: Campanacci, V.]]
+[[Category: Giudici-Orticoni MT]]
-[[Category: Giudici-Orticoni, M T]]
+[[Category: Hallberg BM]]
-[[Category: Hallberg, B M.]]
+[[Category: Jones TA]]
-[[Category: Jones, T A.]]
+[[Category: Lartigue A]]
-[[Category: Lartigue, A.]]
+[[Category: Tegoni M]]
-[[Category: Tegoni, M.]]
-[[Category: Lipid binding protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:14:17 2008''