1n8v: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:


==Chemosensory Protein in complex with bromo-dodecanol==
==Chemosensory Protein in complex with bromo-dodecanol==
<StructureSection load='1n8v' size='340' side='right' caption='[[1n8v]], [[Resolution|resolution]] 1.39&Aring;' scene=''>
<StructureSection load='1n8v' size='340' side='right'caption='[[1n8v]], [[Resolution|resolution]] 1.39&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1n8v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cabbage_moth Cabbage moth]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N8V FirstGlance]. <br>
<table><tr><td colspan='2'>[[1n8v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mamestra_brassicae Mamestra brassicae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N8V FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BDD:BROMO-DODECANOL'>BDD</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.39&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1n8u|1n8u]], [[1kx8|1kx8]], [[1kx9|1kx9]], [[1k19|1k19]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDD:BROMO-DODECANOL'>BDD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n8v OCA], [http://pdbe.org/1n8v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n8v RCSB], [http://www.ebi.ac.uk/pdbsum/1n8v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n8v ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n8v OCA], [https://pdbe.org/1n8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n8v RCSB], [https://www.ebi.ac.uk/pdbsum/1n8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n8v ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9NG96_MAMBR Q9NG96_MAMBR]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Line 18: Line 20:
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n8v ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n8v ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Chemosensory proteins (CSPs) have been proposed to transport hydrophobic chemicals from air to olfactory or taste receptors. They have been isolated from several sensory organs of a wide range of insect species. The x-ray structure of CSPMbraA6, a 112-aa antennal protein from the moth Mamestra brassicae (Mbra), was shown to exhibit a novel type of alpha-helical fold. We have performed a structural and binding study of CSPMbraA6 to get some insights into its possible molecular function. Tryptophan fluorescence quenching demonstrates the ability of CSPMbraA6 to bind several types of semio-chemicals or surrogate ligands with microM K(d). Its crystal structure in complex with one of these compounds, 12-bromo-dodecanol, reveals extensive conformational changes on binding, resulting in the formation of a large cavity filled by three ligand molecules. Furthermore, binding cooperativity was demonstrated for some ligands, suggesting a stepwise binding. The peculiar rearrangement of CSPMbraA6 conformation and the cooperativity phenomenon might trigger the recognition of chemicals by receptors and induce subsequent signal transduction.
Moth chemosensory protein exhibits drastic conformational changes and cooperativity on ligand binding.,Campanacci V, Lartigue A, Hallberg BM, Jones TA, Giudici-Orticoni MT, Tegoni M, Cambillau C Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5069-74. Epub 2003 Apr 15. PMID:12697900<ref>PMID:12697900</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1n8v" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Cabbage moth]]
[[Category: Large Structures]]
[[Category: Cambillau, C]]
[[Category: Mamestra brassicae]]
[[Category: Campanacci, V]]
[[Category: Cambillau C]]
[[Category: Giudici-Orticoni, M T]]
[[Category: Campanacci V]]
[[Category: Hallberg, B M]]
[[Category: Giudici-Orticoni MT]]
[[Category: Jones, T A]]
[[Category: Hallberg BM]]
[[Category: Lartigue, A]]
[[Category: Jones TA]]
[[Category: Tegoni, M]]
[[Category: Lartigue A]]
[[Category: Lipid binding protein]]
[[Category: Tegoni M]]

Latest revision as of 11:47, 10 April 2024

Chemosensory Protein in complex with bromo-dodecanolChemosensory Protein in complex with bromo-dodecanol

Structural highlights

1n8v is a 2 chain structure with sequence from Mamestra brassicae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.39Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9NG96_MAMBR

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1n8v, resolution 1.39Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1n8v&oldid=4125926"