1mcx: Difference between revisions

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New page: left|200px<br /><applet load="1mcx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mcx, resolution 2.03Å" /> '''STRUCTURE OF FULL-LE...
 
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[[Image:1mcx.jpg|left|200px]]<br /><applet load="1mcx" size="450" color="white" frame="true" align="right" spinBox="true"
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'''STRUCTURE OF FULL-LENGTH ANNEXIN A1 IN THE PRESENCE OF CALCIUM'''<br />


==Overview==
==STRUCTURE OF FULL-LENGTH ANNEXIN A1 IN THE PRESENCE OF CALCIUM==
In 1993, Huber and co-workers published the structure of an N-terminally, truncated version of human annexin A1 lacking the first 32 amino acid, residues (PDB code: 1AIN). In 2001, we reported the structure of, full-length porcine annexin A1 including the N-terminal domain in the, absence of calcium ions (PDB code: 1HM6). The latter structure did not, reflect a typical annexin core fold, but rather a surprising interaction, of the N-terminal domain and the core domain. Comparing these two, structures revealed that in the full-length structure the first 12, residues of the N-terminal domain insert into the core of the protein, thereby replacing and unwinding one of the alpha-helices (helix D in, repeat 3) that is involved in calcium binding. We hypothesized that this, structure in the absence of calcium ions represents the inactive form of, the protein. Furthermore, we proposed that upon calcium binding, the, N-terminal domain would be expelled from the core domain and that the core, D-helix would reform in the proper conformation for calcium coordination., Herein, we report the X-ray structure of full-length porcine annexin A1 in, the presence of calcium. This new structure shows a typical annexin core, structure as we hypothesized, with the D-helix back in place for calcium, coordination while parts of the now exposed N-terminal domain are, disordered. We could locate eight calcium ions in this structure, two of, which are octa-coordinated and two of which were not observed in the, structure of the N-terminally truncated annexin A1. Possible implications, of this calcium-induced conformational switch for the membrane aggregation, properties of annexin A1 will be discussed.
<StructureSection load='1mcx' size='340' side='right'caption='[[1mcx]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mcx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MCX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MCX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mcx OCA], [https://pdbe.org/1mcx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mcx RCSB], [https://www.ebi.ac.uk/pdbsum/1mcx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mcx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ANXA1_PIG ANXA1_PIG] Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mc/1mcx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mcx ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1MCX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MCX OCA].
*[[Annexin 3D structures|Annexin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
A calcium-driven conformational switch of the N-terminal and core domains of annexin A1., Rosengarth A, Luecke H, J Mol Biol. 2003 Mar 7;326(5):1317-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12595246 12595246]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
[[Category: Luecke, H.]]
[[Category: Luecke H]]
[[Category: Rosengarth, A.]]
[[Category: Rosengarth A]]
[[Category: CA]]
[[Category: calcium/phospholipid binding protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:20:04 2007''

Latest revision as of 11:34, 10 April 2024

STRUCTURE OF FULL-LENGTH ANNEXIN A1 IN THE PRESENCE OF CALCIUMSTRUCTURE OF FULL-LENGTH ANNEXIN A1 IN THE PRESENCE OF CALCIUM

Structural highlights

1mcx is a 1 chain structure with sequence from Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.03Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ANXA1_PIG Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1mcx, resolution 2.03Å

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