134d: Difference between revisions

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New page: left|200px<br /><applet load="134d" size="450" color="white" frame="true" align="right" spinBox="true" caption="134d" /> '''SOLUTION STRUCTURE OF A PURINE(DOT)PURINE(DO...
 
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[[Image:134d.jpg|left|200px]]<br /><applet load="134d" size="450" color="white" frame="true" align="right" spinBox="true"
caption="134d" />
'''SOLUTION STRUCTURE OF A PURINE(DOT)PURINE(DOT)PYRIMIDINE DNA TRIPLEX CONTAINING G(DOT)GC AND T(DOT)AT TRIPLES'''<br />


==Overview==
==SOLUTION STRUCTURE OF A PURINE(DOT)PURINE(DOT)PYRIMIDINE DNA TRIPLEX CONTAINING G(DOT)GC AND T(DOT)AT TRIPLES==
BACKGROUND: Oligonucleotide-directed triple helix formation allows, sequence specific recognition of double helical DNA. This powerful, approach has been used to inhibit gene transcription in vitro and to, mediate single site specific cleavage of a human chromosome. RESULTS:, Using a combined NMR and molecular dynamics approach (including relaxation, matrix refinement), we have determined the solution structure of an, intramolecular purine.purine.pyrimidine (R.RY) DNA triplex containing, guanines and thymines in the third strand to high resolution. Our studies, define the G.GC and T.AT base triple pairing alignments in the R.RY, triplex and identify the structural discontinuities in the third strand, associated with the non-isomorphism of the base triples. The 5'-d(TpG)-3', base steps exhibit a pronounced increase in axial rise and reduction in, helical twist, while the reverse is observed, to a lesser extent at, 5'-d(GpT)-3' steps. A third groove is formed between the purine-rich third, strand and the pyrimidine strand. It is wider and deeper than the other, two grooves. CONCLUSIONS: Our structure of the R.RY DNA triplex will be, important in the design of oligonucleotide probes with enhanced, specificity and affinity for targeting in the genome. The third groove, presents a potential target for binding additional ligands.
<StructureSection load='134d' size='340' side='right'caption='[[134d]]' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[134d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=134D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=134D FirstGlance]. <br>
134D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=134D OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=134d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=134d OCA], [https://pdbe.org/134d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=134d RCSB], [https://www.ebi.ac.uk/pdbsum/134d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=134d ProSAT]</span></td></tr>
==Reference==
</table>
Solution structure of a purine.purine.pyrimidine DNA triplex containing G.GC and T.AT triples., Radhakrishnan I, Patel DJ, Structure. 1993 Oct 15;1(2):135-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8069626 8069626]
__TOC__
[[Category: Protein complex]]
</StructureSection>
[[Category: Patel, D.J.]]
[[Category: Large Structures]]
[[Category: Radhakrishnan, I.]]
[[Category: Synthetic construct]]
[[Category: dna]]
[[Category: Patel DJ]]
[[Category: nmr]]
[[Category: Radhakrishnan I]]
[[Category: triplex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 21:52:45 2007''

Latest revision as of 11:05, 10 April 2024

SOLUTION STRUCTURE OF A PURINE(DOT)PURINE(DOT)PYRIMIDINE DNA TRIPLEX CONTAINING G(DOT)GC AND T(DOT)AT TRIPLESSOLUTION STRUCTURE OF A PURINE(DOT)PURINE(DOT)PYRIMIDINE DNA TRIPLEX CONTAINING G(DOT)GC AND T(DOT)AT TRIPLES

Structural highlights

134d is a 1 chain structure with sequence from Synthetic construct. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT
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