VprBP: Difference between revisions

<StructureSection load='5jk7' size='400' side='right' caption='Human VprBP residues 1045-1396 (pink) complex with Vpr (cyan), DNA damage-binding protein (green) and uracil-DNA glycosylase (yellow) (PDB code [[5jk7]]' scene='77/776391/Cv/2'>

'''VprBP''' ('''VPR-binding protein''') is a HIV-1 WD40 protein is essential for DNA replication and embryonic development<ref>PMID:18606781</ref>.  VprBP has intrinsic kinase activity and is capable of phosphorylating histone H2A.  Recruitment of VprBP by Vpr is essential for HIV-1 VPR activity of initiating the host cell response cycle arresting its G(2) phase following mitosis<ref>PMID:17314515</ref>.  VprBP interacts with merlin which is then recruited to the E3 ligase complex resulting in its polyubiquitination and consequently its proteasome-mediated degradation<ref>PMID:18332868</ref>.

The interactions between VprBP and Vpr are located in a cleft formed by <scene name='77/776391/Cv/5'>VprBD canonical WD40 seven-blade β-propeller</scene> ({{Template:ColorKey_Helix}}, {{Template:ColorKey_Strand}}, {{Template:ColorKey_Loop}}, {{Template:ColorKey_Turn}}) which is <scene name='77/776391/Cv/6'>lined by acidic residues on one end and hydrophobic residues at the center</scene> ({{Template:ColorKey_Charge_Anionic}} / {{Template:ColorKey_Charge_Cationic}}; {{Template:ColorKey_Hydrophobic}},  {{Template:ColorKey_Polar}}). The interactions are formed by hydrogen bonds, Vpr Phe residue buried in VprBP hydrophobic pocket and VprBP Trp binding to residues in Vpr pocket<ref>PMID:27571178</ref>.

 

 

[[4pxw]] – hVprBP residues 1039-1401 (mutant) - human <br />