1kl8: Difference between revisions

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-[[Image:1kl8.png|left|200px]]
-{{STRUCTURE_1kl8|  PDB=1kl8  |  SCENE=  }}
+==NMR STRUCTURAL ANALYSIS OF THE COMPLEX FORMED BETWEEN ALPHA-BUNGAROTOXIN AND THE PRINCIPAL ALPHA-NEUROTOXIN BINDING SEQUENCE ON THE ALPHA7 SUBUNIT OF A NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR==
+<StructureSection load='1kl8' size='340' side='right'caption='[[1kl8]]' scene=''>
-===NMR STRUCTURAL ANALYSIS OF THE COMPLEX FORMED BETWEEN ALPHA-BUNGAROTOXIN AND THE PRINCIPAL ALPHA-NEUROTOXIN BINDING SEQUENCE ON THE ALPHA7 SUBUNIT OF A NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR===
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>
-{{ABSTRACT_PUBMED_11790782}}
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
-==About this Structure==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl8 OCA], [https://pdbe.org/1kl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kl8 RCSB], [https://www.ebi.ac.uk/pdbsum/1kl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kl8 ProSAT]</span></td></tr>
-[[1kl8]] is a 2 chain structure of [[Bungarotoxin]] with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA].
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/3L21A_BUNMU 3L21A_BUNMU] Binds with high affinity to muscular (tested on Torpedo marmorata, Kd=0.4 nM) and neuronal (tested on chimeric alpha-7/CHRNA7, Kd=0.95 nM) nicotinic acetylcholine receptor (nAChR) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular and neuronal transmission (PubMed:9305882). It also shows an activity on GABA(A) receptors (PubMed:16549768, PubMed:25634239). It antagonises GABA-activated currents with high potency when tested on primary hippocampal neurons (PubMed:25634239). It inhibits recombinantly expressed GABA(A) receptors composed of alpha-2-beta-2-gamma-2 (GABRA2-GABRB2-GABRG2) subunits with high potency (62.3% inhibition at 20 uM of toxin) (PubMed:25634239). It also shows a weaker inhibition on GABA(A) receptors composed of alpha-1-beta-2-gamma-2 (GABRA1-GABRB2-GABRG2) subunits, alpha-4-beta-2-gamma-2 (GABRA4-GABRB2-GABRG2) subunits, and alpha-5-beta-2-gamma-2 (GABRA5-GABRB2-GABRG2) subunits (PubMed:25634239). A very weak inhibition is also observed on GABA(A) receptor composed of alpha-1-beta-3-gamma-2 (GABRA1-GABRB3-GABRG2) (PubMed:26221036). It has also been shown to bind and inhibit recombinant GABA(A) receptor beta-3/GABRB3 subunit (Kd=about 50 nM) (PubMed:16549768). In addition, it blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM) (PubMed:9840221).<ref>PMID:16549768</ref> <ref>PMID:25634239</ref> <ref>PMID:9305882</ref> <ref>PMID:9840221</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/1kl8_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kl8 ConSurf].
+<div style="clear:both"></div>
 ==See Also==
-*[[Bungarotoxin|Bungarotoxin]]
+*[[Bungarotoxin 3D structures|Bungarotoxin 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:011790782</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Bungarus multicinctus]]
 [[Category: Gallus gallus]]
-[[Category: Basus, V J.]]
+[[Category: Large Structures]]
-[[Category: Hawrot, E.]]
+[[Category: Basus VJ]]
-[[Category: Moise, L.]]
+[[Category: Hawrot E]]
-[[Category: Piserchio, A.]]
+[[Category: Moise L]]
-[[Category: Alpha-bungarotoxin]]
+[[Category: Piserchio A]]
-[[Category: Alpha-neurotoxin]]
-[[Category: Ligand-gated ion channel]]
-[[Category: Nicotinic acetylcholine receptor alpha 7 subunit]]
-[[Category: Nmr protein-protein interaction]]
-[[Category: Protein-peptide complex]]
-[[Category: Toxin]]