482d: Difference between revisions

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 ==RELEASE OF THE CYANO MOIETY IN THE CRYSTAL STRUCTURE OF N-CYANOMETHYL-N-(2-METHOXYETHYL)-DAUNOMYCIN COMPLEXED WITH D(CGATCG)==
-<StructureSection load='482d' size='340' side='right' caption='[[482d]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
+<StructureSection load='482d' size='340' side='right'caption='[[482d]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[482d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=482D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=482D FirstGlance]. <br>
+<table><tr><td colspan='2'>[[482d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=482D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=482D FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DM9:N-HYDROXYMETHYL-N-(2-METHOXYETHYL)-DAUNOMYCIN'>DM9</scene><br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=482d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=482d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=482d RCSB], [http://www.ebi.ac.uk/pdbsum/482d PDBsum]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DM9:N-HYDROXYMETHYL-N-(2-METHOXYETHYL)-DAUNOMYCIN'>DM9</scene></td></tr>
-<table>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=482d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=482d OCA], [https://pdbe.org/482d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=482d RCSB], [https://www.ebi.ac.uk/pdbsum/482d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=482d ProSAT]</span></td></tr>
-<div style="background-color:#fffaf0;">
+</table>
-== Publication Abstract from PubMed ==
-Doxorubicin is among the most widely used anthracycline in cancer chemotherapy. In an attempt to avoid the cardiotoxicity and drug resistance of doxorubicin therapy, several analogues were synthesized. The cyanomorpholinyl derivative is the most cytotoxic. They differ greatly from their parent compound in their biological and pharmacological properties, inducing cross-links in drug DNA complexes. The present study concerns N-cyanomethyl-N-(2-methoxyethyl)-daunomycin (CMDa), a synthetic analogue of cyanomorpholino-daunomycin. Compared to doxorubicin, CMDa displays a cytotoxic activity on L1210 leukemia cells at higher concentration but is effective on doxorubicin resistant cells. The results of fluorescence quenching experiments as well as the melting temperature (DeltaTm = 7.5 degrees C) studies are consistent with a drug molecule which intercalates between the DNA base pairs and stabilizes the DNA double helix. The crystal structure of CMDa complexed to the hexanucleotide d(CGATCG) has been determined at 1.5 A resolution. The complex crystallizes in the space group P41212 and is similar to other anthracycline-hexanucleotide complexes. In the crystal state, the observed densities indicate the formation of N-hydroxymethyl-N-(2-methoxyethyl)-daunomycin (HMDa) with the release of the cyano moiety without DNA alkylation. The formation of this degradation compound is discussed in relation with other drug modifications when binding to DNA. Comparison with two other drug-DNA crystal structures suggests a correlation between a slight change in DNA conformation and the nature of the amino sugar substituents at the N3' position located in the minor groove.
-Release of the cyano moiety in the crystal structure of N-cyanomethyl-N-(2-methoxyethyl)-daunomycin complexed with d(CGATCG).,Saminadin P, Dautant A, Mondon M, Langlois D'estaintot B, Courseille C, Precigoux G Eur J Biochem. 2000 Jan;267(2):457-64. PMID:10632715<ref>PMID:10632715</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Courseille, C.]]
+[[Category: Large Structures]]
-[[Category: Dautant, A.]]
+[[Category: Courseille C]]
-[[Category: Estaintot, B Langlois D.]]
+[[Category: Dautant A]]
-[[Category: Mondon, M.]]
+[[Category: Langlois D'Estaintot B]]
-[[Category: Precigoux, G.]]
+[[Category: Mondon M]]
-[[Category: Saminadin, P.]]
+[[Category: Precigoux G]]
-[[Category: Complexed with drug]]
+[[Category: Saminadin P]]
-[[Category: Deoxyribonucleic acid]]
-[[Category: Dna]]
-[[Category: Double helix]]
-[[Category: Right handed dna]]