2oq5: Difference between revisions

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-[[Image:2oq5.gif|left|200px]]<br />
-<applet load="2oq5" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2oq5, resolution 1.61&Aring;" />
-'''Crystal structure of DESC1, a new member of the type II transmembrane serine proteinases family'''<br />
-==Overview==
+==Crystal structure of DESC1, a new member of the type II transmembrane serine proteinases family==
-DESC1 was identified using gene-expression analysis between squamous cell, carcinoma of the head and neck and normal tissue. It belongs to the type, II transmembrane multidomain serine proteinases (TTSPs), an expanding, family of serine proteinases, whose members are differentially expressed, in several tissues. The biological role of these proteins is currently, under investigation, although in some cases their participation in, specific functions has been reported. This is the case for, enteropeptidase, hepsin, matriptase and corin. Some members, including, DESC1, are associated with cell differentiation and have been described as, tumor markers. TTSPs belong to the type II transmembrane proteins that, display, in addition to a C-terminal trypsin-like serine proteinase, domain, a differing set of stem domains, a transmembrane segment and a, short N-terminal cytoplasmic region. Based on sequence analysis, the TTSP, family is subdivided into four subfamilies: hepsin/transmembrane, proteinase, serine (TMPRSS); matriptase; corin; and the human airway, trypsin (HAT)/HAT-like/DESC subfamily. Members of the hepsin and, matriptase subfamilies are known structurally and here we present the, crystal structure of DESC1 as a first member of the HAT/HAT-like/DESC, subfamily in complex with benzamidine. The proteinase domain of DESC1, exhibits a trypsin-like serine proteinase fold with a thrombin-like S1, pocket, a urokinase-type plasminogen activator-type S2 pocket, to accept, small residues, and an open hydrophobic S3/S4 cavity to accept large, hydrophobic residues. The deduced substrate specificity for DESC1 differs, markedly from that of other structurally known TTSPs. Based on surface, analysis, we propose a rigid domain association for the N-terminal SEA, domain with the back site of the proteinase domain.
+<StructureSection load='2oq5' size='340' side='right'caption='[[2oq5]], [[Resolution|resolution]] 1.61&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2oq5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OQ5 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.61&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oq5 OCA], [https://pdbe.org/2oq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oq5 RCSB], [https://www.ebi.ac.uk/pdbsum/2oq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oq5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TM11E_HUMAN TM11E_HUMAN] Serine protease which possesses both gelatinolytic and caseinolytic activities. Shows a preference for Arg in the P1 position (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oq/2oq5_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oq5 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-OQ5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with BEN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2OQ5 OCA].
+*[[Transmembrane protease serine 2|Transmembrane protease serine 2]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family., Kyrieleis OJ, Huber R, Ong E, Oehler R, Hunter M, Madison EL, Jacob U, FEBS J. 2007 Mar 27;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17388811 17388811]
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Huber, R.]]
+[[Category: Huber R]]
-[[Category: Kyrieleis, O.J.P.]]
+[[Category: Jacob U]]
-[[Category: Madison, E.L.]]
+[[Category: Kyrieleis OJP]]
-[[Category: U., Jacob.]]
+[[Category: Madison EL]]
-[[Category: BEN]]
-[[Category: trypsin-like serine protease]]
-[[Category: tumor marker]]
-[[Category: type ii trans-membrane serine proteinases]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:14:55 2007''