2f6a: Difference between revisions

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New page: left|200px<br /><applet load="2f6a" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f6a, resolution 3.29Å" /> '''Collagen Adhesin and...
 
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[[Image:2f6a.jpg|left|200px]]<br /><applet load="2f6a" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2f6a, resolution 3.29&Aring;" />
'''Collagen Adhesin and Collagen Complex Structure'''<br />


==Overview==
==Collagen Adhesin and Collagen Complex Structure==
The structural basis for the association of eukaryotic and prokaryotic, protein receptors and their triple-helical collagen ligand remains poorly, understood. Here, we present the crystal structures of a high affinity, subsegment of the Staphylococcus aureus collagen-binding CNA as an, apo-protein and in complex with a synthetic collagen-like triple helical, peptide. The apo-protein structure is composed of two subdomains (N1 and, N2), each adopting a variant IgG-fold, and a long linker that connects N1, and N2. The structure is stabilized by hydrophobic inter-domain, interactions and by the N2 C-terminal extension that complements a, beta-sheet on N1. In the ligand complex, the collagen-like peptide, penetrates through a spherical hole formed by the two subdomains and the, N1-N2 linker. Based on these two structures we propose a dynamic, multistep binding model, called the 'Collagen Hug' that is uniquely, designed to allow multidomain collagen binding proteins to bind their, extended rope-like ligand.
<StructureSection load='2f6a' size='340' side='right'caption='[[2f6a]], [[Resolution|resolution]] 3.29&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2f6a]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saimiriine_gammaherpesvirus_2 Saimiriine gammaherpesvirus 2] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F6A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F6A FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.29&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f6a OCA], [https://pdbe.org/2f6a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f6a RCSB], [https://www.ebi.ac.uk/pdbsum/2f6a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f6a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CNA_STAAU CNA_STAAU] Mediates attachment of staphylococcal cells to collagen-containing substrata.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f6/2f6a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f6a ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2F6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2F6A OCA].
*[[Adhesin 3D structures|Adhesin 3D structures]]
 
*[[Collagen 3D structures|Collagen 3D structures]]
==Reference==
__TOC__
A 'Collagen Hug' model for Staphylococcus aureus CNA binding to collagen., Zong Y, Xu Y, Liang X, Keene DR, Hook A, Gurusiddappa S, Hook M, Narayana SV, EMBO J. 2005 Dec 21;24(24):4224-36. Epub 2005 Dec 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16362049 16362049]
</StructureSection>
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Saimiriine gammaherpesvirus 2]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
[[Category: Narayana, S.L.V.]]
[[Category: Narayana SLV]]
[[Category: Zong, Y.]]
[[Category: Zong Y]]
[[Category: adhesion]]
[[Category: cna]]
[[Category: collagen]]
[[Category: ecm]]
[[Category: mscramm]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:23:20 2007''

Latest revision as of 09:25, 3 April 2024

Collagen Adhesin and Collagen Complex StructureCollagen Adhesin and Collagen Complex Structure

Structural highlights

2f6a is a 10 chain structure with sequence from Saimiriine gammaherpesvirus 2 and Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.29Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CNA_STAAU Mediates attachment of staphylococcal cells to collagen-containing substrata.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2f6a, resolution 3.29Å

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