2bc3: Difference between revisions

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-[[Image:2bc3.gif|left|200px]]
-<!--
+==T7-tagged full-length streptavidin==
-The line below this paragraph, containing "STRUCTURE_2bc3", creates the "Structure Box" on the page.
+<StructureSection load='2bc3' size='340' side='right'caption='[[2bc3]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2bc3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BC3 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_2bc3|  PDB=2bc3  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bc3 OCA], [https://pdbe.org/2bc3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bc3 RCSB], [https://www.ebi.ac.uk/pdbsum/2bc3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bc3 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bc/2bc3_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bc3 ConSurf].
+<div style="clear:both"></div>
-'''T7-tagged full-length streptavidin'''
+==See Also==
+*[[Avidin 3D structures|Avidin 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-The structure of a full-length streptavidin has been determined at 1.7 A resolution and shows that the 20 residue extension at the C terminus forms a well-ordered polypeptide loop on the surface of the tetramer. Residues 150-153 of the extension are bound to the ligand-binding site, possibly competing with exogenous ligands. The binding mode of these residues is compared with that of biotin and peptidic ligands. The observed structure helps to rationalize the observations that full-length mature streptavidin binds biotinylated macromolecules with reduced affinity.
+[[Category: Large Structures]]
-==About this Structure==
-BC3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BC3 OCA].
-==Reference==
-Crystallographic analysis of a full-length streptavidin with its C-terminal polypeptide bound in the biotin binding site., Le Trong I, Humbert N, Ward TR, Stenkamp RE, J Mol Biol. 2006 Feb 24;356(3):738-45. Epub 2005 Dec 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16384581 16384581]
-[[Category: Single protein]]
 [[Category: Streptomyces avidinii]]
-[[Category: Humbert, N.]]
+[[Category: Humbert N]]
-[[Category: Stenkamp, R E.]]
+[[Category: Le Trong I]]
-[[Category: Trong, I Le.]]
+[[Category: Stenkamp RE]]
-[[Category: Ward, T R.]]
+[[Category: Ward TR]]
-[[Category: Streptavidin]]
-[[Category: T7 tag]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:05:53 2008''