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[[Image:1u9y.gif|left|200px]]


{{Structure
==Crystal Structure of Phosphoribosyl Diphosphate Synthase from Methanocaldococcus jannaschii==
|PDB= 1u9y |SIZE=350|CAPTION= <scene name='initialview01'>1u9y</scene>, resolution 2.65&Aring;
<StructureSection load='1u9y' size='340' side='right'caption='[[1u9y]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1u9y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U9Y FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Ribose-phosphate_diphosphokinase Ribose-phosphate diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.1 2.7.6.1]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
|GENE= prs ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u9y OCA], [https://pdbe.org/1u9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u9y RCSB], [https://www.ebi.ac.uk/pdbsum/1u9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u9y ProSAT]</span></td></tr>
}}
</table>
 
== Function ==
'''Crystal Structure of Phosphoribosyl Diphosphate Synthase from Methanocaldococcus jannaschii'''
[https://www.uniprot.org/uniprot/KPRS_METJA KPRS_METJA]
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
The prs gene encoding phosphoribosyl diphosphate (PRPP) synthase of the hyperthermophilic autotrophic methanogenic archaeon Methanocaldococcus jannaschii has been cloned and expressed in Escherichia coli. Subsequently, M.jannaschii PRPP synthase has been purified, characterised, crystallised, and the crystal structure determined. The enzyme is activated by phosphate ions and only ATP or dATP serve as diphosphoryl donors. The K(m) values are determined as 2.6 mM and 2.8 mM for ATP and ribose 5-phosphate, respectively, and the V(max) value as 2.20 mmol (minxmg of protein)(-1). ADP is a potent inhibitor of activity while GDP has no effect. A single ADP binding site, the active site, is present per subunit. The crystal structure of the enzyme reveals a more compact subunit than that of the enzyme from the mesophile Bacillus subtilis, caused by truncations at the N and C terminus as well as shorter loops in the M.jannaschii enzyme. The M.jannaschii enzyme displays a tetrameric quaternary structure in contrast to the hexameric quaternary structure of B.subtilis PRPP synthase. Soaking of the crystals with 5'-AMP and PRPP revealed the position of the former compound as well as that of ribose 5-phosphate. The properties of M.jannaschii PRPP synthase differ widely from previously characterised PRPP synthases by its tetrameric quaternary structure and the simultaneous phosphate ion-activation and lack of allosteric inhibition, and, thus, constitute a novel class of PRPP synthases.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u9/1u9y_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
1U9Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U9Y OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u9y ConSurf].
Novel class III phosphoribosyl diphosphate synthase: structure and properties of the tetrameric, phosphate-activated, non-allosterically inhibited enzyme from Methanocaldococcus jannaschii., Kadziola A, Jepsen CH, Johansson E, McGuire J, Larsen S, Hove-Jensen B, J Mol Biol. 2005 Dec 9;354(4):815-28. Epub 2005 Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16288921 16288921]
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Ribose-phosphate diphosphokinase]]
[[Category: Hove-Jensen B]]
[[Category: Single protein]]
[[Category: Jepsen CH]]
[[Category: Hove-Jensen, B.]]
[[Category: Johansson E]]
[[Category: Jepsen, C H.]]
[[Category: Kadziola A]]
[[Category: Johansson, E.]]
[[Category: Larsen S]]
[[Category: Kadziola, A.]]
[[Category: McGuire J]]
[[Category: Larsen, S.]]
[[Category: McGuire, J.]]
[[Category: prpp synthase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:29:12 2008''

Latest revision as of 09:16, 3 April 2024

Crystal Structure of Phosphoribosyl Diphosphate Synthase from Methanocaldococcus jannaschiiCrystal Structure of Phosphoribosyl Diphosphate Synthase from Methanocaldococcus jannaschii

Structural highlights

1u9y is a 4 chain structure with sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.65Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KPRS_METJA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1u9y, resolution 2.65Å

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OCA
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