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[[Image:1phz.jpg|left|200px]]


{{Structure
==STRUCTURE OF PHOSPHORYLATED PHENYLALANINE HYDROXYLASE==
|PDB= 1phz |SIZE=350|CAPTION= <scene name='initialview01'>1phz</scene>, resolution 2.20&Aring;
<StructureSection load='1phz' size='340' side='right'caption='[[1phz]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>
<table><tr><td colspan='2'>[[1phz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The January 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phenylalanine Hydroxylase''  by Shuchismita Dutta and David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_1 10.2210/rcsb_pdb/mom_2005_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PHZ FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1phz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1phz OCA], [https://pdbe.org/1phz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1phz RCSB], [https://www.ebi.ac.uk/pdbsum/1phz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1phz ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1phz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1phz OCA], [http://www.ebi.ac.uk/pdbsum/1phz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1phz RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/PH4H_RAT PH4H_RAT]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ph/1phz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1phz ConSurf].
<div style="clear:both"></div>


'''STRUCTURE OF PHOSPHORYLATED PHENYLALANINE HYDROXYLASE'''
==See Also==
 
*[[Hydroxylases 3D structures|Hydroxylases 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Phenylalanine hydroxylase converts phenylalanine to tyrosine, a rate-limiting step in phenylalanine catabolism and protein and neurotransmitter biosynthesis. It is tightly regulated by the substrates phenylalanine and tetrahydrobiopterin and by phosphorylation. We present the crystal structures of dephosphorylated and phosphorylated forms of a dimeric enzyme with catalytic and regulatory properties of the wild-type protein. The structures reveal a catalytic domain flexibly linked to a regulatory domain. The latter consists of an N-terminal autoregulatory sequence (containing Ser 16, which is the site of phosphorylation) that extends over the active site pocket, and an alpha-beta sandwich core that is, unexpectedly, structurally related to both pterin dehydratase and the regulatory domains of metabolic enzymes. Phosphorylation has no major structural effects in the absence of phenylalanine, suggesting that phenylalanine and phosphorylation act in concert to activate the enzyme through a combination of intrasteric and possibly allosteric mechanisms.
[[Category: Large Structures]]
 
==About this Structure==
1PHZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The following page contains interesting information on the relation of 1PHZ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb61_1.html Phenylalanine Hydroxylase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PHZ OCA].
 
==Reference==
Structural basis of autoregulation of phenylalanine hydroxylase., Kobe B, Jennings IG, House CM, Michell BJ, Goodwill KE, Santarsiero BD, Stevens RC, Cotton RG, Kemp BE, Nat Struct Biol. 1999 May;6(5):442-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10331871 10331871]
[[Category: Phenylalanine 4-monooxygenase]]
[[Category: Phenylalanine Hydroxylase]]
[[Category: Phenylalanine Hydroxylase]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Cotton RG]]
[[Category: Cotton, R G.]]
[[Category: House CM]]
[[Category: House, C M.]]
[[Category: Jennings IG]]
[[Category: Jennings, I G.]]
[[Category: Kemp BE]]
[[Category: Kemp, B E.]]
[[Category: Kobe B]]
[[Category: Kobe, B.]]
[[Category: Michell BJ]]
[[Category: Michell, B J.]]
[[Category: allosteric regulation]]
[[Category: aromatic amino acid hydroxylase]]
[[Category: intrasteric regulation]]
[[Category: phenylalanine hydroxylase]]
[[Category: phosphorylation]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:59:53 2008''

Latest revision as of 09:10, 3 April 2024

STRUCTURE OF PHOSPHORYLATED PHENYLALANINE HYDROXYLASESTRUCTURE OF PHOSPHORYLATED PHENYLALANINE HYDROXYLASE

Structural highlights

1phz is a 1 chain structure with sequence from Rattus norvegicus. The January 2005 RCSB PDB Molecule of the Month feature on Phenylalanine Hydroxylase by Shuchismita Dutta and David S. Goodsell is 10.2210/rcsb_pdb/mom_2005_1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PH4H_RAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1phz, resolution 2.20Å

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