1mvb: Difference between revisions

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-[[Image:1mvb.gif|left|200px]]
-<!--
+==STRUCTURE OF A PROTEIN CAPSID OF THE T59S MUTANT OF PHAGE MS2==
-The line below this paragraph, containing "STRUCTURE_1mvb", creates the "Structure Box" on the page.
+<StructureSection load='1mvb' size='340' side='right'caption='[[1mvb]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_MS2 Escherichia phage MS2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MVB FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [https://pdbe.org/1mvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [https://www.ebi.ac.uk/pdbsum/1mvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvb ProSAT]</span></td></tr>
-{{STRUCTURE_1mvb|  PDB=1mvb  |  SCENE=  }}
+</table>
+== Function ==
-'''STRUCTURE OF A PROTEIN CAPSID OF THE T59S MUTANT OF PHAGE MS2'''
+[https://www.uniprot.org/uniprot/CAPSD_BPMS2 CAPSD_BPMS2] Self-assembles to form the T=3 icosahedral virus shell that protects the viral nucleic acid. Acts as a translational repressor by binding with high specificity to a single stem-loop structure in the genomic RNA that contains the initiation codon of the gene for the viral replicase. Involved in virus assembly through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome.<ref>PMID:16531233</ref> <ref>PMID:18662904</ref> <ref>PMID:26608810</ref> <ref>PMID:8254664</ref> <ref>PMID:9245600</ref> <ref>PMID:9469847</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
-==Overview==
+Check<jmol>
-In MS2 assembly of phage particles results from an interaction between a coat protein dimer and a stem-loop of the RNA genome (the operator hairpin). Amino acid residues Thr45, which is universally conserved among the small RNA phages, and Thr59 are part of the specific RNA binding pocket and interact directly with the RNA; the former through a hydrogen bond, the latter through hydrophobic contacts. The crystal structures of MS2 protein capsids formed by mutants Thr45Ala and Thr59Ser, both with and without the 19 nt wild-type operator hairpin bound, are reported here. The RNA hairpin binds to these mutants in a similar way to its binding to wild-type protein. In a companion paper both mutants are shown to be deficient in RNA binding in an in vivo assay, but in vitro the equilibrium dissociation constant is significantly higher than wild-type for the Thr45Ala mutant. The change in binding affinity of the Thr45Ala mutant is probably a direct consequence of removal of direct hydrogen bonds between the protein and the RNA. The properties of the Thr59Ser mutant are more difficult to explain, but are consistent with a loss of non-polar contact.
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/1mvb_consurf.spt"</scriptWhenChecked>
-==About this Structure==
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-MVB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA].
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
-==Reference==
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mvb ConSurf].
-Crystal structures of MS2 coat protein mutants in complex with wild-type RNA operator fragments., van den Worm SH, Stonehouse NJ, Valegard K, Murray JB, Walton C, Fridborg K, Stockley PG, Liljas L, Nucleic Acids Res. 1998 Mar 1;26(5):1345-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9469847 9469847]
+<div style="clear:both"></div>
-[[Category: Enterobacterio phage ms2]]
+== References ==
-[[Category: Single protein]]
+<references/>
-[[Category: Liljas, L.]]
+__TOC__
-[[Category: Stonehouse, N J.]]
+</StructureSection>
-[[Category: Valegard, K.]]
+[[Category: Escherichia phage MS2]]
-[[Category: Vandenworm, S.]]
+[[Category: Large Structures]]
-[[Category: Bacteriophage coat protein]]
+[[Category: Liljas L]]
-[[Category: Icosahedral virus]]
+[[Category: Stonehouse NJ]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:45:50 2008''
+[[Category: Valegard K]]
+[[Category: Vandenworm S]]