1dnv: Difference between revisions

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New page: left|200px<br /><applet load="1dnv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dnv, resolution 3.6Å" /> '''PARVOVIRUS (DENSOVIRU...
 
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'''PARVOVIRUS (DENSOVIRUS) FROM GALLERIA MELLONELLA'''<br />


==Overview==
==PARVOVIRUS (DENSOVIRUS) FROM GALLERIA MELLONELLA==
BACKGROUND: Parvoviruses infect vertebrates, insects and crustaceans. Many, arthropod parvoviruses (densoviruses) are highly pathogenic and kill, approximately 90% of the host larvae within days, making them potentially, effective as selective pesticides. Improved understanding of densoviral, structure and function is therefore desirable. There are four different, initiation sites for translation of the densovirus capsid protein mRNA, giving rise to the viral proteins VP1 to VP4. Sixty copies of the common, C-terminal domain make up the ordered part of the icosahedral capsid., RESULTS: The Galleria mellonella densovirus (GMDNV) capsid protein, consists of a core beta-barrel motif, similar to that found in many other, viral capsid proteins. The structure most closely resembles that of the, vertebrate parvoviruses, but it has diverged beyond recognition in many of, the long loop regions that constitute the surface features and, intersubunit contacts. The N termini of twofold-related subunits have, swapped their positions relative to those of the vertebrate parvoviruses., Unlike in the vertebrate parvoviruses, in GmDNV there is no continuous, electron density in the channels running along the fivefold axes of the, virus. Electron density corresponding to some of the single-stranded DNA, genome is visible in the crystal structure, but it is not as well defined, as in the vertebrate parvoviruses. CONCLUSIONS: The sequence of the, glycine-rich motif, which occupies each of the channels along the fivefold, axes in vertebrate viruses, is conserved between mammalian and insect, parvoviruses. This motif may serve to externalize the N-terminal region of, the single VP1 subunit per particle. The domain swapping of the N termini, between insect and vertebrate parvoviruses may have the effect of, increasing capsid stability in GmDNV.
<StructureSection load='1dnv' size='340' side='right'caption='[[1dnv]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1dnv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Galleria_mellonella_densovirus Galleria mellonella densovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DNV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DNV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dnv OCA], [https://pdbe.org/1dnv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dnv RCSB], [https://www.ebi.ac.uk/pdbsum/1dnv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dnv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAPSD_GMDNV CAPSD_GMDNV] Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of size variants of the capsid proteins, which differ in the N-terminushe capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dn/1dnv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dnv ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1DNV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Galleria_mellonella_densovirus Galleria mellonella densovirus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DNV OCA].
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The structure of an insect parvovirus (Galleria mellonella densovirus) at 3.7 A resolution., Simpson AA, Chipman PR, Baker TS, Tijssen P, Rossmann MG, Structure. 1998 Nov 15;6(11):1355-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9817847 9817847]
[[Category: Galleria mellonella densovirus]]
[[Category: Galleria mellonella densovirus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Baker, T.S.]]
[[Category: Baker TS]]
[[Category: Chipmann, P.R.]]
[[Category: Chipmann PR]]
[[Category: Rossmann, M.G.]]
[[Category: Rossmann MG]]
[[Category: Simpson, A.A.]]
[[Category: Simpson AA]]
[[Category: Tijssen, P.]]
[[Category: Tijssen P]]
[[Category: capsid protein]]
[[Category: densovirus]]
[[Category: icosahedral virus]]
[[Category: parvovirus]]
[[Category: viral capsid]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:25:56 2007''

Latest revision as of 08:58, 3 April 2024

PARVOVIRUS (DENSOVIRUS) FROM GALLERIA MELLONELLAPARVOVIRUS (DENSOVIRUS) FROM GALLERIA MELLONELLA

Structural highlights

1dnv is a 1 chain structure with sequence from Galleria mellonella densovirus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAPSD_GMDNV Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of size variants of the capsid proteins, which differ in the N-terminushe capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1dnv, resolution 3.60Å

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