1hg0: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /> <applet load="1hg0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hg0, resolution 1.90Å" /> '''X-RAY STRUCTURE OF ...
 
No edit summary
 
(23 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1hg0.gif|left|200px]]<br />
<applet load="1hg0" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1hg0, resolution 1.90&Aring;" />
'''X-RAY STRUCTURE OF THE COMPLEX BETWEEN ERWINIA CHRYSANTHEMI L-ASPARAGINASE AND SUCCINIC ACID'''<br />


==Overview==
==X-ray structure of the complex between Erwinia chrysanthemi L-asparaginase and succinic acid==
Bacterial L-asparaginases, enzymes that catalyze the hydrolysis of, L-asparagine to aspartic acid, have been used for over 30 years as, therapeutic agents in the treatment of acute childhood lymphoblastic, leukemia. Other substrates of asparaginases include L-glutamine, D-asparagine, and succinic acid monoamide. In this report, we present, high-resolution crystal structures of the complexes of Erwinia, chrysanthemi L-asparaginase (ErA) with the products of such reactions that, also can serve as substrates, namely L-glutamic acid (L-Glu), D-aspartic, acid (D-Asp), and succinic acid (Suc). Comparison of the four independent, active sites within each complex indicates unique and specific binding of, the ligand molecules; the mode of binding is also similar between, complexes. The lack of the ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11341830 (full description)]]
<StructureSection load='1hg0' size='340' side='right'caption='[[1hg0]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1hg0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HG0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hg0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hg0 OCA], [https://pdbe.org/1hg0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hg0 RCSB], [https://www.ebi.ac.uk/pdbsum/1hg0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hg0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ASPG_DICCH ASPG_DICCH]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hg/1hg0_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hg0 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1HG0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]] with SIN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HG0 OCA]].
*[[Asparaginase 3D structures|Asparaginase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structural basis for the activity and substrate specificity of Erwinia chrysanthemi L-asparaginase., Aghaiypour K, Wlodawer A, Lubkowski J, Biochemistry. 2001 May 15;40(19):5655-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11341830 11341830]
[[Category: Dickeya chrysanthemi]]
[[Category: Erwinia chrysanthemi]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Kolyani KA]]
[[Category: Kolyani, K.A.]]
[[Category: Lubkowski J]]
[[Category: Lubkowski, J.]]
[[Category: Wlodawer A]]
[[Category: Wlodawer, A.]]
[[Category: SIN]]
[[Category: asparaginase]]
[[Category: complex]]
[[Category: d-aspartate]]
[[Category: hydrolase]]
[[Category: structure]]
[[Category: x-ray]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 16:08:08 2007''

Latest revision as of 14:31, 27 March 2024

X-ray structure of the complex between Erwinia chrysanthemi L-asparaginase and succinic acidX-ray structure of the complex between Erwinia chrysanthemi L-asparaginase and succinic acid

Structural highlights

1hg0 is a 4 chain structure with sequence from Dickeya chrysanthemi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ASPG_DICCH

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1hg0, resolution 1.90Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1hg0&oldid=4115229"