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[[Image:1hfj.gif|left|200px]]<br /><applet load="1hfj" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1hfj, resolution 2.40&Aring;" />
'''ASPARAGINASE FROM ERWINIA CHRYSANTHEMI, HEXAGONAL FORM WITH SULFATE'''<br />


==Overview==
==Asparaginase from Erwinia chrysanthemi, hexagonal form with sulfate==
Quasi-enantiomorphic crystals of the Y25F mutant of Escherichia coli, L-asparaginase and of the native Erwinia chrysanthemi L-asparaginase were, obtained in the hexagonal space groups P6(5)22 and P6(1)22, respectively., The structures of these highly homologous enzymes were solved by molecular, replacement and were refined with data extending to 2.2-2.5 A. These, structures were compared with each other, as well as with other, L-asparaginase structures previously observed with different crystal, packing. It is concluded that the observed phenomenon, which is rare, was, most likely to have arisen by chance.
<StructureSection load='1hfj' size='340' side='right'caption='[[1hfj]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1hfj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HFJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hfj OCA], [https://pdbe.org/1hfj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hfj RCSB], [https://www.ebi.ac.uk/pdbsum/1hfj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hfj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ASPG_DICCH ASPG_DICCH]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hf/1hfj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hfj ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1HFJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] Known structural/functional Sites: <scene name='pdbsite=AS1:Active Site Chain A'>AS1</scene> and <scene name='pdbsite=AS2:Active Site Chain C'>AS2</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HFJ OCA].
*[[Asparaginase 3D structures|Asparaginase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structures of two highly homologous bacterial L-asparaginases: a case of enantiomorphic space groups., Jaskolski M, Kozak M, Lubkowski J, Palm G, Wlodawer A, Acta Crystallogr D Biol Crystallogr. 2001 Mar;57(Pt 3):369-77. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11223513 11223513]
[[Category: Dickeya chrysanthemi]]
[[Category: Asparaginase]]
[[Category: Large Structures]]
[[Category: Erwinia chrysanthemi]]
[[Category: Jaskolski M]]
[[Category: Single protein]]
[[Category: Kozak M]]
[[Category: Jaskolski, M.]]
[[Category: Lubkowski J]]
[[Category: Kozak, M.]]
[[Category: Palm GJ]]
[[Category: Lubkowski, J.]]
[[Category: Wlodawer A]]
[[Category: Palm, G.J.]]
[[Category: Wlodawer, A.]]
[[Category: SO4]]
[[Category: hydrolase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 16:21:08 2007''

Latest revision as of 14:31, 27 March 2024

Asparaginase from Erwinia chrysanthemi, hexagonal form with sulfateAsparaginase from Erwinia chrysanthemi, hexagonal form with sulfate

Structural highlights

1hfj is a 2 chain structure with sequence from Dickeya chrysanthemi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ASPG_DICCH

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1hfj, resolution 2.40Å

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