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[[Image:1gpx.gif|left|200px]]<br />
<applet load="1gpx" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1gpx" />
'''C85S GAPDX, NMR, 20 STRUCTURES'''<br />


==Overview==
==C85S GAPDX, NMR, 20 STRUCTURES==
The Fe2S2 cluster of the ferredoxin putidaredoxin (Pdx) can be replaced by, a single gallium ion, giving rise to a colorless, diamagnetic protein in, which, apart from the metal binding site, the major structural features of, the native ferredoxin are conserved. The solution structure of the C85S, variant of gallium putidaredoxin (C85S GaPdx), in which a non-ligand, cysteine is replaced by a serine, has been determined via multidimensional, NMR methods using uniformly 15N, 13C labeled samples of C85S GaPdx., Stereospecific assignments of leucine and valine methyl resonances were, made using 13C, 1H HSQC spectra obtained with fractionally 13C-labeled, samples, and backbone dihedral angle restraints were obtained using a, combination of two-dimensional J-modulated 15N, 1H HSQC and, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9835048 (full description)]]
<StructureSection load='1gpx' size='340' side='right'caption='[[1gpx]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1gpx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GPX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GA:GALLIUM+(III)+ION'>GA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpx OCA], [https://pdbe.org/1gpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gpx RCSB], [https://www.ebi.ac.uk/pdbsum/1gpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gpx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PUTX_PSEPU PUTX_PSEPU] The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gp/1gpx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gpx ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1GPX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with GA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: GAL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GPX OCA]].
*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The solution structure of a gallium-substituted putidaredoxin mutant: GaPdx C85S., Pochapsky TC, Kuti M, Kazanis S, J Biomol NMR. 1998 Oct;12(3):407-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9835048 9835048]
[[Category: Large Structures]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
[[Category: Single protein]]
[[Category: Kazanis S]]
[[Category: Kazanis, S.]]
[[Category: Kuti M]]
[[Category: Kuti, M.]]
[[Category: Pochapsky TC]]
[[Category: Pochapsky, T.C.]]
[[Category: GA]]
[[Category: 20 structures aligned and sa]]
[[Category: electron transport]]
[[Category: gapdx c85s]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:16:55 2007''

Latest revision as of 14:23, 27 March 2024

C85S GAPDX, NMR, 20 STRUCTURESC85S GAPDX, NMR, 20 STRUCTURES

Structural highlights

1gpx is a 1 chain structure with sequence from Pseudomonas putida. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PUTX_PSEPU The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

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