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[[Image:1gjw.jpg|left|200px]]<br /><applet load="1gjw" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1gjw, resolution 2.1&Aring;" />
'''THERMOTOGA MARITIMA MALTOSYLTRANSFERASE COMPLEX WITH MALTOSE'''<br />


==Overview==
==Thermotoga maritima maltosyltransferase complex with maltose==
Maltosyltransferase (MTase) from the hyperthermophile Thermotoga maritima represents a novel maltodextrin glycosyltransferase acting on starch and malto-oligosaccharides. It catalyzes the transfer of maltosyl units from alpha-1,4-linked glucans or malto-oligosaccharides to other alpha-1,4-linked glucans, malto-oligosaccharides or glucose. It belongs to the glycoside hydrolase family 13, which represents a large group of (beta/alpha)(8) barrel proteins sharing a similar active site structure. The crystal structures of MTase and its complex with maltose have been determined at 2.4 A and 2.1 A resolution, respectively. MTase is a homodimer, each subunit of which consists of four domains, two of which are structurally homologous to those of other family 13 enzymes. The catalytic core domain has the (beta/alpha)(8) barrel fold with the active-site cleft formed at the C-terminal end of the barrel. Substrate binding experiments have led to the location of two distinct maltose-binding sites; one lies in the active-site cleft, covering subsites -2 and -1; the other is located in a pocket adjacent to the active-site cleft. The structure of MTase, together with the conservation of active-site residues among family 13 glycoside hydrolases, are consistent with a common double-displacement catalytic mechanism for this enzyme. Analysis of maltose binding in the active site reveals that the transfer of dextrinyl residues longer than a maltosyl unit is prevented by termination of the active-site cleft after the -2 subsite by the side-chain of Lys151 and the stretch of residues 314-317, providing an explanation for the strict transfer specificity of MTase.
<StructureSection load='1gjw' size='340' side='right'caption='[[1gjw]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1gjw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GJW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjw OCA], [https://pdbe.org/1gjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gjw RCSB], [https://www.ebi.ac.uk/pdbsum/1gjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gjw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O33838_THEMT O33838_THEMT]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/1gjw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gjw ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1GJW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with <scene name='pdbligand=MAL:'>MAL</scene>, <scene name='pdbligand=GLC:'>GLC</scene> and <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=GLC:Glc+Binding+Site+For+Chain+A'>GLC</scene> and <scene name='pdbsite=MAL:Mal+Binding+Site+For+Chain+A'>MAL</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJW OCA].
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The crystal structure of Thermotoga maritima maltosyltransferase and its implications for the molecular basis of the novel transfer specificity., Roujeinikova A, Raasch C, Burke J, Baker PJ, Liebl W, Rice DW, J Mol Biol. 2001 Sep 7;312(1):119-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11545590 11545590]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Thermotoga maritima MSB8]]
[[Category: Thermotoga maritima]]
[[Category: Baker PJ]]
[[Category: Baker, P J.]]
[[Category: Burke J]]
[[Category: Burke, J.]]
[[Category: Liebl W]]
[[Category: Liebl, W.]]
[[Category: Raasch C]]
[[Category: Raasch, C.]]
[[Category: Rice DW]]
[[Category: Rice, D W.]]
[[Category: Roujeinikova A]]
[[Category: Roujeinikova, A.]]
[[Category: GLC]]
[[Category: MAL]]
[[Category: PO4]]
[[Category: alpha-amylase]]
[[Category: maltosyltransferase]]
[[Category: transferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:50:55 2008''

Latest revision as of 14:21, 27 March 2024

Thermotoga maritima maltosyltransferase complex with maltoseThermotoga maritima maltosyltransferase complex with maltose

Structural highlights

1gjw is a 1 chain structure with sequence from Thermotoga maritima MSB8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O33838_THEMT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1gjw, resolution 2.10Å

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