1fwd: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(20 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1fwd.gif|left|200px]]<br />
<applet load="1fwd" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1fwd, resolution 2.0&Aring;" />
'''KLEBSIELLA AEROGENES UREASE, C319A VARIANT AT PH 9.4'''<br />


==Overview==
==KLEBSIELLA AEROGENES UREASE, C319A VARIANT AT PH 9.4==
Cys319 is located on a mobile flap covering the active site of Klebsiella, aerogenes urease but does not play an essential role in catalysis. Four, urease variants altered at position C319 range from having high activity, (C319A) to no measurable activity (C319Y), indicating Cys is not required, at this position, but its presence is highly influential [Martin, P. R., &amp;, Hausinger, R. P. (1992) J. Biol. Chem. 267, 20024-20027]. Here, we present, 2.0 A resolution crystal structures of C319A, C319S, C319D, and C319Y, proteins and the C319A variant inhibited by acetohydroxamic acid. These, structures show changes in the hydration of the active site nickel ions, and in the position and flexibility of the active site flap. The C319Y, protein exhibits an alternate conformation of the flap, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9201965 (full description)]]
<StructureSection load='1fwd' size='340' side='right'caption='[[1fwd]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1fwd]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FWD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FWD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fwd OCA], [https://pdbe.org/1fwd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fwd RCSB], [https://www.ebi.ac.uk/pdbsum/1fwd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fwd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/URE3_KLEAE URE3_KLEAE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fw/1fwd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fwd ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1FWD is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]] with NI as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Urease Urease]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5]]. Structure known Active Sites: ACT and NIL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FWD OCA]].
*[[Urease 3D structures|Urease 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structures of Cys319 variants and acetohydroxamate-inhibited Klebsiella aerogenes urease., Pearson MA, Michel LO, Hausinger RP, Karplus PA, Biochemistry. 1997 Jul 1;36(26):8164-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9201965 9201965]
[[Category: Klebsiella aerogenes]]
[[Category: Klebsiella aerogenes]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Urease]]
[[Category: Karplus PA]]
[[Category: Karplus, P.A.]]
[[Category: Pearson MA]]
[[Category: Pearson, M.A.]]
[[Category: NI]]
[[Category: hydrolase(urea amido)]]
[[Category: mutant]]
[[Category: nickel metalloenzyme]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:13:07 2007''

Latest revision as of 14:17, 27 March 2024

KLEBSIELLA AEROGENES UREASE, C319A VARIANT AT PH 9.4KLEBSIELLA AEROGENES UREASE, C319A VARIANT AT PH 9.4

Structural highlights

1fwd is a 3 chain structure with sequence from Klebsiella aerogenes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

URE3_KLEAE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1fwd, resolution 2.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1fwd&oldid=4114057"