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[[Image:1fno.gif|left|200px]]


{{Structure
==PEPTIDASE T (TRIPEPTIDASE)==
|PDB= 1fno |SIZE=350|CAPTION= <scene name='initialview01'>1fno</scene>, resolution 2.4&Aring;
<StructureSection load='1fno' size='340' side='right'caption='[[1fno]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
<table><tr><td colspan='2'>[[1fno]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FNO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FNO FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fno OCA], [https://pdbe.org/1fno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fno RCSB], [https://www.ebi.ac.uk/pdbsum/1fno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fno ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fno OCA], [http://www.ebi.ac.uk/pdbsum/1fno PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fno RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/PEPT_SALTY PEPT_SALTY] Cleaves the N-terminal amino acid of tripeptides. Hydrolyzes tripeptides containing N-terminal methionine, leucine, or phenylalanine. Displays little or no activity against dipeptides, N-blocked or C-blocked tripeptides, and tetrapeptides.<ref>PMID:6341363</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fn/1fno_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fno ConSurf].
<div style="clear:both"></div>


'''PEPTIDASE T (TRIPEPTIDASE)'''
==See Also==
 
*[[Peptidase T|Peptidase T]]
 
== References ==
==Overview==
<references/>
The structure of peptidase T, or tripeptidase, was determined by multiple wavelength anomalous dispersion (MAD) methodology and refined to 2.4 A resolution. Peptidase T comprises two domains; a catalytic domain with an active site containing two metal ions, and a smaller domain formed through a long insertion into the catalytic domain. The two metal ions, presumably zinc, are separated by 3.3 A, and are coordinated by five carboxylate and histidine ligands. The molecular surface of the active site is negatively charged. Peptidase T has the same basic fold as carboxypeptidase G2. When the structures of the two enzymes are superimposed, a number of homologous residues, not evident from the sequence alone, could be identified. Comparison of the active sites of peptidase T, carboxypeptidase G2, Aeromonas proteolytica aminopeptidase, carboxypeptidase A and leucine aminopeptidase reveals a common structural framework with interesting similarities and differences in the active sites and in the zinc coordination. A putative binding site for the C-terminal end of the tripeptide substrate was found at a peptidase T specific fingerprint sequence motif.
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
1FNO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FNO OCA].
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
 
[[Category: Hakansson K]]
==Reference==
[[Category: Miller CG]]
Structure of peptidase T from Salmonella typhimurium., Hakansson K, Miller CG, Eur J Biochem. 2002 Jan;269(2):443-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11856302 11856302]
[[Category: Salmonella typhimurium]]
[[Category: Single protein]]
[[Category: Hakansson, K.]]
[[Category: Miller, C G.]]
[[Category: metallo peptidase]]
[[Category: protease]]
[[Category: zinc]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:26:34 2008''

Latest revision as of 14:14, 27 March 2024

PEPTIDASE T (TRIPEPTIDASE)PEPTIDASE T (TRIPEPTIDASE)

Structural highlights

1fno is a 1 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PEPT_SALTY Cleaves the N-terminal amino acid of tripeptides. Hydrolyzes tripeptides containing N-terminal methionine, leucine, or phenylalanine. Displays little or no activity against dipeptides, N-blocked or C-blocked tripeptides, and tetrapeptides.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Strauch KL, Miller CG. Isolation and characterization Salmonella typhimurium mutants lacking a tripeptidase (peptidase T). J Bacteriol. 1983 May;154(2):763-71. PMID:6341363

1fno, resolution 2.40Å

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