1fj8: Difference between revisions

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-[[Image:1fj8.jpg|left|200px]]<br /><applet load="1fj8" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1fj8, resolution 2.27&Aring;" />
-'''THE STRUCTURE OF BETA-KETOACYL-[ACYL CARRIER PROTEIN] SYNTHASE I IN COMPLEX WITH CERULENIN, IMPLICATIONS FOR DRUG DESIGN'''<br />
-==Overview==
+==THE STRUCTURE OF BETA-KETOACYL-[ACYL CARRIER PROTEIN] SYNTHASE I IN COMPLEX WITH CERULENIN, IMPLICATIONS FOR DRUG DESIGN==
-The beta-ketoacyl-acyl carrier protein (ACP) synthases are key regulators, of type II fatty acid synthesis and are the targets for two natural, products, thiolactomycin (TLM) and cerulenin. The high resolution, structures of the FabB-TLM and FabB-cerulenin binary complexes were, determined. TLM mimics malonyl-ACP in the FabB active site. It forms, strong hydrogen bond interactions with the two catalytic histidines, and, the unsaturated alkyl side chain interaction with a small hydrophobic, pocket is stabilized by pi stacking interactions. Cerulenin binding mimics, the condensation transition state. The subtle differences between the, FabB-cerulenin and FabF-cerulenin (Moche, M., Schneider, G., Edwards, P., Dehesh, K., and Lindqvist, Y. (1999) J. Biol. Chem. 244, 6031-6034), structures explain the differences in the sensitivity of the two enzymes, to the antibiotic and may reflect the distinct substrate specificities, that differentiate the two enzymes. The FabB[H333N] protein was prepared, to convert the FabB His-His-Cys active site triad into the FabH, His-Asn-Cys configuration to test the importance of the two His residues, in TLM and cerulenin binding. FabB[H333N] was significantly more resistant, to both antibiotics than FabB and had an affinity for TLM an order of, magnitude less than the wild-type enzyme, illustrating that the, two-histidine active site architecture is critical to protein-antibiotic, interaction. These data provide a structural framework for understanding, antibiotic sensitivity within this group of enzymes.
+<StructureSection load='1fj8' size='340' side='right'caption='[[1fj8]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1fj8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FJ8 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CER:(2S,+3R)-3-HYDROXY-4-OXO-7,10-TRANS,TRANS-DODECADIENAMIDE'>CER</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fj8 OCA], [https://pdbe.org/1fj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fj8 RCSB], [https://www.ebi.ac.uk/pdbsum/1fj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fj8 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FABB_ECOLI FABB_ECOLI] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-10 to unsaturated C-16 and C-18 fatty acids.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/1fj8_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fj8 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-FJ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CER as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Beta-ketoacyl-acyl-carrier-protein_synthase_I Beta-ketoacyl-acyl-carrier-protein synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FJ8 OCA].
+*[[Acyl carrier protein synthase 3D structures|Acyl carrier protein synthase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Inhibition of beta-ketoacyl-acyl carrier protein synthases by thiolactomycin and cerulenin. Structure and mechanism., Price AC, Choi KH, Heath RJ, Li Z, White SW, Rock CO, J Biol Chem. 2001 Mar 2;276(9):6551-9. Epub 2000 Oct 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11050088 11050088]
-[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]
 [[Category: Escherichia coli]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Choi, K.]]
+[[Category: Choi K]]
-[[Category: Heath, R.J.]]
+[[Category: Heath RJ]]
-[[Category: Li, Z.]]
+[[Category: Li Z]]
-[[Category: Price, A.C.]]
+[[Category: Price AC]]
-[[Category: Rock, C.O.]]
+[[Category: Rock CO]]
-[[Category: White, S.W.]]
+[[Category: White SW]]
-[[Category: CER]]
-[[Category: cerulenin]]
-[[Category: condensing enzymes]]
-[[Category: drug design]]
-[[Category: fatty acid elongation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:58:01 2007''