7dd3: Difference between revisions

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====
==Cryo-EM structure of the pre-mRNA-loaded DEAH-box ATPase/helicase Prp2 in complex with Spp2==
<StructureSection load='7dd3' size='340' side='right'caption='[[7dd3]]' scene=''>
<StructureSection load='7dd3' size='340' side='right'caption='[[7dd3]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
<table><tr><td colspan='2'>[[7dd3]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DD3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DD3 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7dd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dd3 OCA], [http://pdbe.org/7dd3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7dd3 RCSB], [http://www.ebi.ac.uk/pdbsum/7dd3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7dd3 ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dd3 OCA], [https://pdbe.org/7dd3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dd3 RCSB], [https://www.ebi.ac.uk/pdbsum/7dd3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dd3 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PRP2_YEAST PRP2_YEAST] Involved in pre-mRNA splicing. Is required together with ATP and at least one other factor, for the first cleavage-ligation reaction. Functions as a molecular motor in the activation of the precatalytic spliceosome for the first transesterification reaction of pre-mRNA splicing by hydrolyzing ATP to cause the activation of the spliceosome without the occurrence of splicing. Capable of hydrolyzing nucleoside triphosphates in the presence of single-stranded RNAs such as poly(U).<ref>PMID:14730020</ref> <ref>PMID:1534753</ref> <ref>PMID:2251118</ref> <ref>PMID:8112302</ref> <ref>PMID:8943336</ref>
==See Also==
*[[Helicase 3D structures|Helicase 3D structures]]
*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Z-disk]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Bai R]]
[[Category: Lei J]]
[[Category: Qi J]]
[[Category: Shi Y]]
[[Category: Wan R]]
[[Category: Yan C]]
[[Category: Zhang P]]

Latest revision as of 13:53, 27 March 2024

Cryo-EM structure of the pre-mRNA-loaded DEAH-box ATPase/helicase Prp2 in complex with Spp2Cryo-EM structure of the pre-mRNA-loaded DEAH-box ATPase/helicase Prp2 in complex with Spp2

Structural highlights

7dd3 is a 3 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PRP2_YEAST Involved in pre-mRNA splicing. Is required together with ATP and at least one other factor, for the first cleavage-ligation reaction. Functions as a molecular motor in the activation of the precatalytic spliceosome for the first transesterification reaction of pre-mRNA splicing by hydrolyzing ATP to cause the activation of the spliceosome without the occurrence of splicing. Capable of hydrolyzing nucleoside triphosphates in the presence of single-stranded RNAs such as poly(U).[1] [2] [3] [4] [5]

See Also

References

  1. ↑ Edwalds-Gilbert G, Kim DH, Silverman E, Lin RJ. Definition of a spliceosome interaction domain in yeast Prp2 ATPase. RNA. 2004 Feb;10(2):210-20. PMID:14730020
  2. ↑ Kim SH, Smith J, Claude A, Lin RJ. The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase. EMBO J. 1992 Jun;11(6):2319-26. PMID:1534753
  3. ↑ King DS, Beggs JD. Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae. Nucleic Acids Res. 1990 Nov 25;18(22):6559-64. PMID:2251118
  4. ↑ Teigelkamp S, McGarvey M, Plumpton M, Beggs JD. The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA. EMBO J. 1994 Feb 15;13(4):888-97. PMID:8112302
  5. ↑ Kim SH, Lin RJ. Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing. Mol Cell Biol. 1996 Dec;16(12):6810-9. PMID:8943336

7dd3, resolution 3.20Å

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