7cnb: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:


==Crystal structure of Gp16 C-terminal domain from Bacillus virus phi29==
==Crystal structure of Gp16 C-terminal domain from Bacillus virus phi29==
<StructureSection load='7cnb' size='340' side='right'caption='[[7cnb]]' scene=''>
<StructureSection load='7cnb' size='340' side='right'caption='[[7cnb]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CNB FirstGlance]. <br>
<table><tr><td colspan='2'>[[7cnb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_phi29 Bacillus virus phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CNB FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cnb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cnb OCA], [https://pdbe.org/7cnb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cnb RCSB], [https://www.ebi.ac.uk/pdbsum/7cnb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cnb ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.32&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cnb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cnb OCA], [https://pdbe.org/7cnb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cnb RCSB], [https://www.ebi.ac.uk/pdbsum/7cnb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cnb ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PKG16_BPPH2 PKG16_BPPH2] ATPase required for the genome encapsidation reaction. Part of the active packaging motor via the binding to the packaging RNA (pRNA), itself fixed to the head-tail connector at the unique portal vertex of the prohead. Binds and supercoils the DNA-gp3 to produce an initiation complex for DNA packaging. Provides the energy to actively pump the viral DNA into the prohead. Approximately one molecule of ATP is used in the packaging of 2 bp of viral DNA. After packaging, the ATPase and the pRNA are released from the prohead.<ref>PMID:11130079</ref> <ref>PMID:18674782</ref> <ref>PMID:2960820</ref> <ref>PMID:3879485</ref>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus virus phi29]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Ouyang SY]]
[[Category: Ouyang SY]]
[[Category: Saeed AFUH]]
[[Category: Saeed AFUH]]

Latest revision as of 13:51, 27 March 2024

Crystal structure of Gp16 C-terminal domain from Bacillus virus phi29Crystal structure of Gp16 C-terminal domain from Bacillus virus phi29

Structural highlights

7cnb is a 1 chain structure with sequence from Bacillus virus phi29. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.32Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PKG16_BPPH2 ATPase required for the genome encapsidation reaction. Part of the active packaging motor via the binding to the packaging RNA (pRNA), itself fixed to the head-tail connector at the unique portal vertex of the prohead. Binds and supercoils the DNA-gp3 to produce an initiation complex for DNA packaging. Provides the energy to actively pump the viral DNA into the prohead. Approximately one molecule of ATP is used in the packaging of 2 bp of viral DNA. After packaging, the ATPase and the pRNA are released from the prohead.[1] [2] [3] [4]

References

  1. ↑ Simpson AA, Tao Y, Leiman PG, Badasso MO, He Y, Jardine PJ, Olson NH, Morais MC, Grimes S, Anderson DL, Baker TS, Rossmann MG. Structure of the bacteriophage phi29 DNA packaging motor. Nature. 2000 Dec 7;408(6813):745-50. PMID:11130079 doi:10.1038/35047129
  2. ↑ Koti JS, Morais MC, Rajagopal R, Owen BA, McMurray CT, Anderson DL. DNA packaging motor assembly intermediate of bacteriophage phi29. J Mol Biol. 2008 Sep 19;381(5):1114-32. doi: 10.1016/j.jmb.2008.04.034. Epub 2008, Apr 20. PMID:18674782 doi:http://dx.doi.org/10.1016/j.jmb.2008.04.034
  3. ↑ Guo P, Peterson C, Anderson D. Prohead and DNA-gp3-dependent ATPase activity of the DNA packaging protein gp16 of bacteriophage phi 29. J Mol Biol. 1987 Sep 20;197(2):229-36. doi: 10.1016/0022-2836(87)90121-5. PMID:2960820 doi:http://dx.doi.org/10.1016/0022-2836(87)90121-5
  4. ↑ Garvey KJ, Saedi MS, Ito J. The complete sequence of Bacillus phage phi 29 gene 16: a protein required for the genome encapsidation reaction. Gene. 1985;40(2-3):311-6. PMID:3879485

7cnb, resolution 2.32Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=7cnb&oldid=4113540"