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==Cryo-EM structure of multidrug efflux pump MexAB-OprM (60 degree state)==
==Cryo-EM structure of multidrug efflux pump MexAB-OprM (60 degree state)==
<StructureSection load='6iol' size='340' side='right'caption='[[6iol]], [[Resolution|resolution]] 3.76&Aring;' scene=''>
<SX load='6iol' size='340' side='right' viewer='molstar' caption='[[6iol]], [[Resolution|resolution]] 3.76&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6iol]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IOL FirstGlance]. <br>
<table><tr><td colspan='2'>[[6iol]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IOL FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iol OCA], [http://pdbe.org/6iol PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iol RCSB], [http://www.ebi.ac.uk/pdbsum/6iol PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iol ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.76&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iol OCA], [https://pdbe.org/6iol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iol RCSB], [https://www.ebi.ac.uk/pdbsum/6iol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iol ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/MEXA_PSEAE MEXA_PSEAE]] The periplasmic linker component of the MexAB-OprM efflux system that confers multidrug resistance. Also functions as the major efflux pump for n-hexane and p-xylene efflux. Over-expression of the pump increases antibiotic and solvent efflux capacities. Required for assembly of the MexA/MexB/OprM complex. Implicated in the secretion of the siderophore pyoverdine.<ref>PMID:8226684</ref> <ref>PMID:8540696</ref> <ref>PMID:9603892</ref>  The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the MexB subunit is a proton antiporter.<ref>PMID:8226684</ref> <ref>PMID:8540696</ref> <ref>PMID:9603892</ref> [[http://www.uniprot.org/uniprot/MEXB_PSEAE MEXB_PSEAE]] The inner membrane transporter component of the MexAB-OprM efflux system that confers multidrug resistance. Also functions as the major efflux pump for n-hexane and p-xylene efflux. Over-expression of the pump increases antibiotic and solvent efflux capacities. Implicated in the secretion of the siderophore pyoverdine.<ref>PMID:8226684</ref> <ref>PMID:8540696</ref> <ref>PMID:9603892</ref>  The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the MexB subunit is a proton antiporter.<ref>PMID:8226684</ref> <ref>PMID:8540696</ref> <ref>PMID:9603892</ref>  [[http://www.uniprot.org/uniprot/OPRM_PSEAE OPRM_PSEAE]] The outer membrane component of the MexAB-OprM efflux system that confers multidrug resistance. Also functions as the major efflux pump for n-hexane and p-xylene efflux. Over-expression of the pump increases antibiotic and solvent efflux capacities. Can replace the OprJ outer membrane component of the MexCD-OprJ pump; the antibiotics exported are those exported by the intact MexCD pump, showing that efflux substrate specificity is not conferred by this component. Serves as the outer membrane component for the MexXY efflux system. Implicated in the secretion of the siderophore pyoverdine. OprM is probably involved in the efflux of the siderophore across the outer membrane.<ref>PMID:8226684</ref> <ref>PMID:8540696</ref> <ref>PMID:9401051</ref> <ref>PMID:9603892</ref> <ref>PMID:10952562</ref>  The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the MexB subunit is a proton antiporter.<ref>PMID:8226684</ref> <ref>PMID:8540696</ref> <ref>PMID:9401051</ref> <ref>PMID:9603892</ref> <ref>PMID:10952562</ref> 
[https://www.uniprot.org/uniprot/MEXA_PSEAE MEXA_PSEAE] The periplasmic linker component of the MexAB-OprM efflux system that confers multidrug resistance. Also functions as the major efflux pump for n-hexane and p-xylene efflux. Over-expression of the pump increases antibiotic and solvent efflux capacities. Required for assembly of the MexA/MexB/OprM complex. Implicated in the secretion of the siderophore pyoverdine.<ref>PMID:8226684</ref> <ref>PMID:8540696</ref> <ref>PMID:9603892</ref>  The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the MexB subunit is a proton antiporter.<ref>PMID:8226684</ref> <ref>PMID:8540696</ref> <ref>PMID:9603892</ref>  
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Nakagawa, A]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Tsutsumi, K]]
[[Category: Nakagawa A]]
[[Category: Yamashita, E]]
[[Category: Tsutsumi K]]
[[Category: Yonehara, R]]
[[Category: Yamashita E]]
[[Category: Complex]]
[[Category: Yonehara R]]
[[Category: Efflux]]
[[Category: Membrane protein]]
[[Category: Multidrug resistance]]

Latest revision as of 13:34, 27 March 2024

Cryo-EM structure of multidrug efflux pump MexAB-OprM (60 degree state)Cryo-EM structure of multidrug efflux pump MexAB-OprM (60 degree state)

6iol, resolution 3.76Å

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