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| ==Complex of Snf2-Nucleosome complex with Snf2 bound to position +6 of the nucleosome== | | ==Complex of Snf2-Nucleosome complex with Snf2 bound to position +6 of the nucleosome== |
| <StructureSection load='5x0x' size='340' side='right'caption='[[5x0x]], [[Resolution|resolution]] 3.97Å' scene=''> | | <SX load='5x0x' size='340' side='right' viewer='molstar' caption='[[5x0x]], [[Resolution|resolution]] 3.97Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[5x0x]] is a 11 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog] and [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X0X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X0X FirstGlance]. <br> | | <table><tr><td colspan='2'>[[5x0x]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C], [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X0X FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5x0y|5x0y]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.97Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hist1h2aj, LOC494591 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog]), SNF2, GAM1, RIC1, SWI2, TYE3, YOR290C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x0x OCA], [https://pdbe.org/5x0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x0x RCSB], [https://www.ebi.ac.uk/pdbsum/5x0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x0x ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x0x OCA], [http://pdbe.org/5x0x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x0x RCSB], [http://www.ebi.ac.uk/pdbsum/5x0x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x0x ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/H2B11_XENLA H2B11_XENLA]] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [[http://www.uniprot.org/uniprot/H32_XENLA H32_XENLA]] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [[http://www.uniprot.org/uniprot/SNF2_YEAST SNF2_YEAST]] Involved in transcriptional activation. Catalytic component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, which is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors. [[http://www.uniprot.org/uniprot/H4_XENLA H4_XENLA]] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | | [https://www.uniprot.org/uniprot/H32_XENLA H32_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Chromatin remodellers are helicase-like, ATP-dependent enzymes that alter chromatin structure and nucleosome positions to allow regulatory proteins access to DNA. Here we report the cryo-electron microscopy structure of chromatin remodeller Switch/sucrose non-fermentable (SWI2/SNF2) from Saccharomyces cerevisiae bound to the nucleosome. The structure shows that the two core domains of Snf2 are realigned upon nucleosome binding, suggesting activation of the enzyme. The core domains contact each other through two induced Brace helices, which are crucial for coupling ATP hydrolysis to chromatin remodelling. Snf2 binds to the phosphate backbones of one DNA gyre of the nucleosome mainly through its helicase motifs within the major domain cleft, suggesting a conserved mechanism of substrate engagement across different remodellers. Snf2 contacts the second DNA gyre via a positively charged surface, providing a mechanism to anchor the remodeller at a fixed position of the nucleosome. Snf2 locally deforms nucleosomal DNA at the site of binding, priming the substrate for the remodelling reaction. Together, these findings provide mechanistic insights into chromatin remodelling.
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| Mechanism of chromatin remodelling revealed by the Snf2-nucleosome structure.,Liu X, Li M, Xia X, Li X, Chen Z Nature. 2017 Apr 27;544(7651):440-445. doi: 10.1038/nature22036. Epub 2017 Apr, 19. PMID:28424519<ref>PMID:28424519</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 5x0x" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| | *[[Helicase 3D structures|Helicase 3D structures]] |
| *[[Histone 3D structures|Histone 3D structures]] | | *[[Histone 3D structures|Histone 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </SX> |
| [[Category: African clawed frog]]
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| [[Category: Baker's yeast]]
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| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Chen, Z]] | | [[Category: Saccharomyces cerevisiae S288C]] |
| [[Category: Li, M]] | | [[Category: Synthetic construct]] |
| [[Category: Li, X]] | | [[Category: Xenopus laevis]] |
| [[Category: Liu, X]] | | [[Category: Chen Z]] |
| [[Category: Xia, X]] | | [[Category: Li M]] |
| [[Category: Chromatin remodeling]] | | [[Category: Li X]] |
| [[Category: Nucleosome]] | | [[Category: Liu X]] |
| [[Category: Snf2]] | | [[Category: Xia X]] |
| [[Category: Structural protein-hydrolase-dna complex]]
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