Lactoperoxidase: Difference between revisions
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<StructureSection load='3eri' size='350' side='right' caption='Glycosylated bovine lactoferrin containing heme complex with thiocyanate, I- (purple) and Ca+2 (green) ions (PDB entry [[3eri]])' scene='48/487283/Cv/1'> | |||
<StructureSection load='3eri' size='350' side='right' caption=' | |||
== Function == | == Function == | ||
'''Lactoperoxidase''' (LPO) catalyzes the oxidation of thiocyanate, bromide and iodide using hydrogen peroxide. LPO is the second most abundant enzyme in milk. Heme is the cofactor of LPO. LPO contains a strongly-chelated calcium ion<ref>PMID:10837362</ref>. | '''Lactoperoxidase''' (LPO) catalyzes the oxidation of thiocyanate, bromide and iodide using hydrogen peroxide. LPO is the second most abundant enzyme in milk. Heme is the cofactor of LPO. LPO contains a strongly-chelated calcium ion<ref>PMID:10837362</ref>. | ||
== Relevance == | == Relevance == | ||
The short-lived oxidized intermediates of the LPO reaction serve as potent bactericidal agents. LPO is used as an antimicrobial agent in milk and its products, in cosmetics, toothpaste and ophthalmic solutions. | The short-lived oxidized intermediates of the LPO reaction serve as potent bactericidal agents<ref>PMID:10837362</ref>. LPO is used as an antimicrobial agent in milk and its products, in cosmetics, toothpaste and ophthalmic solutions. | ||
== Structural highlights == | |||
The <scene name='48/487283/Cv/6'>peroxidation of thiocyanate catalyzed by LPO occurs at the heme cavity site</scene><ref>PMID:19339248</ref>. Water molecules shown as red spheres. <scene name='48/487283/Cv/7'>Whole active site</scene>. | |||
</StructureSection> | |||
==3D structures of lactoperoxidase== | ==3D structures of lactoperoxidase== | ||
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**[[2pt3]] - bPLO + phosphate - bovine<br /> | **[[2pt3]] - bPLO + phosphate - bovine<br /> | ||
**[[2nqx]] – bPLO + I<br /> | **[[2nqx]], [[5b72]] – bPLO + I<br /> | ||
**[[7dlq]] – bPLO + H2O2<br /> | |||
**[[2r5l]] – gLPO + I – goat<br /> | **[[2r5l]] – gLPO + I – goat<br /> | ||
**[[3nak]] – gLPO + formate<br /> | **[[3nak]] – gLPO + formate<br /> | ||
**[[ | **[[6ky7]], [[7c73]], [[7c74]], [[7c75]], [[7d52]], [[7dao]], [[7de5]], [[7dmr]] – yPLO + Ca - yak<br /> | ||
**[[7c74]] - yLPO + Cl <br /> | |||
**[[6l32]], [[6l42]], [[7d52]], [[6l5g]], [[7c75]] - yLPO + Zn <br /> | |||
**[[6lqw]], [[7y3u]] – yPLO + H2O2<br /> | |||
**[[7c73]] – yPLO + phosphoserine<br /> | |||
*Lactoperoxidase ternary complex | *Lactoperoxidase ternary complex | ||
**[[ | **[[2ips]], [[3eri]], [[3gc1]], [[6a4y]], [[6lco]], [[6m7e]], [[7dn7]] – bLPO + SCN + I<br /> | ||
**[[2qrb]] – bLPO + SCN + Cl<br /> | **[[2qrb]] – bLPO + SCN + Cl<br /> | ||
**[[3bxi]] – bLPO + SCN + nitrate<br /> | **[[3bxi]] – bLPO + SCN + nitrate<br /> | ||
**[[4pnx]] - bPLO + bromomethane + I<br /> | **[[4pnx]] - bPLO + bromomethane + I<br /> | ||
**[[2pum]] – bPLO + catechol + I<br /> | **[[2pum]] – bPLO + catechol + I<br /> | ||
**[[3krq]] – bLPO + amino-triazole + I<br /> | **[[3krq]] – bLPO + amino-triazole + I<br /> | ||
**[[6kmk]] – bPLO + H2O2 + phosphoserine<br /> | |||
**[[7dlq]], [[7dn6]] – bPLO + H2O2 + I<br /> | |||
**[[2z5z]] – wbPLO + F + I – water buffalo<br /> | **[[2z5z]] – wbPLO + F + I – water buffalo<br /> | ||
**[[2o86]] – wbPLO + nitrate + I<br /> | **[[2o86]] – wbPLO + nitrate + I<br /> | ||
**[[3erh]] - wbLPO + SCN + I<br /> | **[[3erh]] - wbLPO + SCN + I<br /> | ||
**[[7de5]] - yLPO + SCN + I<br /> | |||
**[[2ojv]] – gPLO + CN + I<br /> | **[[2ojv]] – gPLO + CN + I<br /> | ||
**[[3n8f]] - gLPO + SCN + nitrate<br /> | **[[3n8f]] - gLPO + SCN + nitrate<br /> | ||
**[[5ff1]] - gLPO + methimazole + nitrate<br /> | |||
**[[5hpw]] - gLPO + propyl-thiouracil + nitrate<br /> | |||
**[[3niu]] – gLPO + diethylene glycol + phosphate<br /> | **[[3niu]] – gLPO + diethylene glycol + phosphate<br /> | ||
**[[3rke]] - gPLO + inhibitor + I<br /> | **[[3rke]] - gPLO + inhibitor + I<br /> | ||
**[[2ikc]] – sLPO + carbonate + formate – sheep | **[[2ikc]] – sLPO + carbonate + formate – sheep<br /> | ||
**[[7ve3]] - sLPO + hypoiodite + I<br /> | |||
*Lactoperoxidase quaternary complex | *Lactoperoxidase quaternary complex | ||
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**[[3i6n]] - bPLO + isoniazid + SCN + I<br /> | **[[3i6n]] - bPLO + isoniazid + SCN + I<br /> | ||
**[[4ksz]] - bPLO + cystein + SCN + I<br /> | **[[4ksz]] - bPLO + cystein + SCN + I<br /> | ||
**[[ | **[[5wv3]] - bPLO + oxidosulfanyl-methanamine + Br + I<br /> | ||
**[[ | **[[7dlq]] - bPLO + Br + Cl + SCN + I<br /> | ||
**[[3py4]], [[ | **[[7wyj]] - bPLO + indomethacin + SCN + I<br /> | ||
**[[ | **[[3py4]], [[6m7e]] - bPLO + paracetamol + SCN + I<br /> | ||
**[[ | **[[7dn6]] - bPLO + phenyliso-SCN + SCN + I<br /> | ||
**[[ | **[[7dn7]] - bPLO + nitro-phenoxymethanesulfonanilide+ SCN + I<br /> | ||
**[[3r4x]], [[ | **[[8y9x]], [[4njb]], [[6lco]] - bPLO + pyridine derivative + SCN + I<br /> | ||
**[[3r4x]], [[5zgs]] - bPLO + pyrazine derivative + SCN + I<br /> | |||
**[[3r5o]] - bPLO + methoxyphenol + SCN + I<br /> | **[[3r5o]] - bPLO + methoxyphenol + SCN + I<br /> | ||
**[[3tgy]] - bPLO + ascorbate + SCN + I<br /> | **[[3tgy]] - bPLO + ascorbate + SCN + I<br /> | ||
**[[3v6q]] - bPLO + CO + SCN + I<br /> | **[[3v6q]] - bPLO + CO + SCN + I<br /> | ||
**[[6l9t]] - bLPO + Zn + OSCN + I<br /> | |||
**[[5gls]] - bLPO + butyl-triazole + SCN + I<br /> | |||
**[[5gh0]] - bLPO + mercaptoimidazole + nitrate + carbonate<br /> | |||
**[[2gjm]] - wbPLO + carbonate + SCN + I<br /> | **[[2gjm]] - wbPLO + carbonate + SCN + I<br /> | ||
**[[3faq]] – wbLPO + CN + SCN + I<br /> | **[[3faq]] – wbLPO + CN + SCN + I<br /> | ||
**[[3fnl]] - wbPLO + salicylhydroxamic acid + SCN + I<br /> | **[[3fnl]] - wbPLO + salicylhydroxamic acid + SCN + I<br /> | ||
**[[4y55]] - wbLPO + rhodanide + SCN + I<br /> | |||
**[[2e9e]] – gPLO + nitrate + carbonate + SCN + CN<br /> | **[[2e9e]] – gPLO + nitrate + carbonate + SCN + CN<br /> | ||
**[[2efb]] – gPLO + phosphate + carbonate + SCN + CN<br /> | **[[2efb]] – gPLO + phosphate + carbonate + SCN + CN<br /> | ||
**[[3qf1]] - gPLO + piperazine + SCN + I<br /> | **[[3qf1]] - gPLO + piperazine + SCN + I<br /> | ||
**[[ | **[[8ing]] - gPLO + acrylonitrile + SCN + I<br /> | ||
**[[4msf]], [[4oek]] - gPLO + phenol derivative+ SCN + I<br /> | **[[4msf]], [[4oek]] - gPLO + phenol derivative+ SCN + I<br /> | ||
**[[2eha]] – gPLO + formate + carbonate + SCN<br /> | **[[2eha]] – gPLO + formate + carbonate + SCN<br /> | ||
**[[3r55]], [[ | **[[3r55]], [[6lf7]] - gPLO + pyrazine derivative + SCN + I<br /> | ||
**[[3sxv]] - gPLO + amitrole + SCN + I<br /> | **[[3sxv]] - gPLO + amitrole + SCN + I<br /> | ||
**[[4qjq]] - gPLO + octopamine + SCN + I<br /> | **[[4qjq]] - gPLO + octopamine + SCN + I<br /> | ||
**[[ | **[[6lf7]] - gPLO + hypocyanite + SCN + H2O2 + I<br /> | ||
**[[ | **[[7ve3]] - sPLO +tetrahydrofuran + SCN + I<br /> | ||
**[[6ky7]], [[6l2j]] – yPLO + H2O2 + phosphoserine + SCN + I<br /> | |||
}} | }} | ||