Lactoperoxidase: Difference between revisions

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Michal Harel, Alexander Berchansky, Joel L. Sussman, Jaime Prilusky

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-{{STRUCTURE_2qqt|  PDB=2qqt  | SIZE=400| SCENE= |right|CAPTION=Heme containing bovine lactoperoxidase complex with aspirin, thiocyanate, calcium and iodide ions [[2qqt]] }}
+<StructureSection load='3eri' size='350' side='right' caption='Glycosylated bovine lactoferrin containing heme complex with thiocyanate, I- (purple) and Ca+2 (green) ions  (PDB entry [[3eri]])' scene='48/487283/Cv/1'>
+== Function ==
+'''Lactoperoxidase''' (LPO) catalyzes the oxidation of thiocyanate, bromide and iodide using hydrogen peroxide.  LPO is the second most abundant enzyme in milk.  Heme is the cofactor of LPO.  LPO contains a strongly-chelated calcium ion<ref>PMID:10837362</ref>.
-<!--
+== Relevance ==
-Please use the "3D" button above this box to insert a Jmol applet (molecule) on this page.
+The short-lived oxidized intermediates of the LPO reaction serve as potent bactericidal agents<ref>PMID:10837362</ref>.  LPO is used as an antimicrobial agent in milk and its products, in cosmetics, toothpaste and ophthalmic solutions.
-Or use the four-green-boxes-button to insert scrollable text adjacent
-to a Jmol applet. Check out the other buttons as well!
+== Structural highlights ==
--->
+The <scene name='48/487283/Cv/6'>peroxidation of thiocyanate catalyzed by LPO occurs at the heme cavity site</scene><ref>PMID:19339248</ref>. Water molecules shown as red spheres. <scene name='48/487283/Cv/7'>Whole active site</scene>.
-'''Lactoperoxidase''' (LPO) catalyzes the oxidation of thiocyanate, bromide and iodide using hydrogen peroxide.  The short-lived oxidized intermediates of the reaction serve as potent bactericidal agents.  LPO is used as an antimicrobial agent in milk and its products, in cosmetics, toothpaste and ophthalmic solutions.  LPO is the second most abundant enzyme in milk.  Heme is the cofactor of LPO.  LPO contains a strongly-chelated calcium ion.
+</StructureSection>
 ==3D structures of lactoperoxidase==
-'''Lactoperoxidase binary complex'''
+Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+{{#tree:id=OrganizedByTopic|openlevels=0|
+*Lactoperoxidase binary complex
+**[[2pt3]] - bPLO + phosphate - bovine<br />
+**[[2nqx]], [[5b72]] – bPLO + I<br />
+**[[7dlq]] – bPLO + H2O2<br />
+**[[2r5l]] – gLPO + I – goat<br />
+**[[3nak]] – gLPO + formate<br />
+**[[6ky7]], [[7c73]], [[7c74]], [[7c75]], [[7d52]], [[7dao]], [[7de5]], [[7dmr]] – yPLO + Ca - yak<br />
+**[[7c74]] - yLPO + Cl <br />
+**[[6l32]], [[6l42]], [[7d52]], [[6l5g]], [[7c75]] - yLPO + Zn <br />
+**[[6lqw]], [[7y3u]] – yPLO + H2O2<br />
+**[[7c73]] – yPLO + phosphoserine<br />
-[[2pt3]] - bPLO + phosphate - bovine<br />
+*Lactoperoxidase ternary complex
-[[2r5l]] – gLPO + I – goat<br />
-[[3nak]] – gLPO + formate
-'''Lactoperoxidase ternary complex'''
+**[[2ips]], [[3eri]], [[3gc1]], [[6a4y]], [[6lco]], [[6m7e]], [[7dn7]] – bLPO + SCN + I<br />
+**[[2qrb]] – bLPO + SCN + Cl<br />
+**[[3bxi]] – bLPO + SCN + nitrate<br />
+**[[4pnx]] - bPLO + bromomethane + I<br />
+**[[2pum]] – bPLO + catechol + I<br />
+**[[3krq]] – bLPO + amino-triazole + I<br />
+**[[6kmk]] – bPLO + H2O2 + phosphoserine<br />
+**[[7dlq]], [[7dn6]] – bPLO + H2O2 + I<br />
+**[[2z5z]] – wbPLO + F + I – water buffalo<br />
+**[[2o86]] – wbPLO + nitrate + I<br />
+**[[3erh]] - wbLPO + SCN + I<br />
+**[[7de5]] - yLPO + SCN + I<br />
+**[[2ojv]] – gPLO + CN + I<br />
+**[[3n8f]] - gLPO + SCN + nitrate<br />
+**[[5ff1]] - gLPO + methimazole + nitrate<br />
+**[[5hpw]] - gLPO + propyl-thiouracil + nitrate<br />
+**[[3niu]] – gLPO + diethylene glycol + phosphate<br />
+**[[3rke]] - gPLO + inhibitor + I<br />
+**[[2ikc]] – sLPO + carbonate + formate – sheep<br />
+**[[7ve3]] - sLPO + hypoiodite + I<br />
-[[2gj1]] – bLPO + carbonate + nitrate <br />
+*Lactoperoxidase quaternary complex
-[[2ips]], [[3eri]], [[3gc1]] – bLPO + SCN + I<br />
-[[2qrb]] – bLPO + SCN + Cl<br />
-[[3bxi]] – bLPO + SCN + nitrate<br />
-[[2pum]] – bPLO + catechol + I<br />
-[[2gj1]] – bLPO + carbonate + nitrate <br />
-[[3krq]] – bLPO + amino-triazole + I<br />
-[[2z5z]] – wbPLO + F + I – water buffalo<br />
-[[2o86]] – wbPLO + nitrate + I<br />
-[[3erh]] - wbLPO + SCN + I<br />
-[[2ojv ]]– gPLO + CN + I<br />
-[[3n8f]] - gLPO + SCN + nitrate<br />
-[[3niu]] – gLPO + diethylene glycol + phosphate<br />
-[[3rke]] - gPLO + inhibitor + I<br />
-[[2ikc]] – sLPO + carbonate + formate – sheep
-'''Lactoperoxidase quaternary complex'''
+**[[3gcj]], [[2qpk]] - bPLO + salicylhydroxamic acid + SCN + I<br />
+**[[3gck]] - bPLO + benzylhydroxamic acid + SCN + I<br />
+**[[3uba]], [[4gm7]] - bPLO + hydroxycinnamic acid + SCN + I<br />
+**[[3gcl]], [[2qqt]] - bPLO + aspirin + SCN + I<br />
+**[[3i6n]] - bPLO + isoniazid + SCN + I<br />
+**[[4ksz]] - bPLO + cystein + SCN + I<br />
+**[[5wv3]] - bPLO + oxidosulfanyl-methanamine + Br + I<br />
+**[[7dlq]] - bPLO + Br + Cl + SCN + I<br />
+**[[7wyj]] - bPLO + indomethacin + SCN + I<br />
+**[[3py4]], [[6m7e]] - bPLO + paracetamol + SCN + I<br />
+**[[7dn6]] - bPLO + phenyliso-SCN + SCN + I<br />
+**[[7dn7]] - bPLO + nitro-phenoxymethanesulfonanilide+ SCN + I<br />
+**[[8y9x]], [[4njb]], [[6lco]] - bPLO + pyridine derivative + SCN + I<br />
+**[[3r4x]], [[5zgs]] - bPLO + pyrazine derivative + SCN + I<br />
+**[[3r5o]] - bPLO + methoxyphenol + SCN + I<br />
+**[[3tgy]] - bPLO + ascorbate + SCN + I<br />
+**[[3v6q]] - bPLO + CO + SCN + I<br />
+**[[6l9t]] - bLPO + Zn + OSCN + I<br />
+**[[5gls]] - bLPO + butyl-triazole + SCN + I<br />
+**[[5gh0]] - bLPO + mercaptoimidazole + nitrate + carbonate<br />
+**[[2gjm]] - wbPLO + carbonate + SCN + I<br />
+**[[3faq]] – wbLPO + CN + SCN + I<br />
+**[[3fnl]] - wbPLO + salicylhydroxamic acid + SCN + I<br />
+**[[4y55]] - wbLPO + rhodanide + SCN + I<br />
+**[[2e9e]] – gPLO + nitrate + carbonate + SCN + CN<br />
+**[[2efb]] – gPLO + phosphate + carbonate + SCN + CN<br />
+**[[3qf1]] - gPLO + piperazine + SCN + I<br />
+**[[8ing]] - gPLO + acrylonitrile + SCN + I<br />
+**[[4msf]], [[4oek]] - gPLO + phenol derivative+ SCN + I<br />
+**[[2eha]] – gPLO + formate + carbonate + SCN<br />
+**[[3r55]], [[6lf7]] - gPLO + pyrazine derivative + SCN + I<br />
+**[[3sxv]] - gPLO + amitrole + SCN + I<br />
+**[[4qjq]] - gPLO + octopamine + SCN + I<br />
+**[[6lf7]] - gPLO + hypocyanite + SCN + H2O2 + I<br />
+**[[7ve3]] - sPLO +tetrahydrofuran + SCN + I<br />
+**[[6ky7]], [[6l2j]] – yPLO + H2O2 + phosphoserine + SCN + I<br />
-[[3gcj]], [[2qpk]] - bPLO + salicylhydroxamic acid + SCN + I<br />
+}}
-[[3gck]] - bPLO + benzylhydroxamic acid + SCN + I<br />
+== References ==
-[[3uba]] - bPLO + hydroxycinnamic acid + SCN + I<br />
+<references/>
-[[3gcl]], [[2qqt]] - bPLO + aspirin + SCN + I<br />
+[[Category:Topic Page]]
-[[3i6n]] - bPLO + isoniazid + SCN + I<br />
-[[3nyh]] - bPLO + Br + Cl + SCN + I<br />
-[[3ogw]] - bPLO + indomethacin + SCN + I<br />
-[[3py4]] - bPLO + paracetamol + SCN + I<br />
-[[3q9k]] - bPLO + phenyliso-SCN + SCN + I<br />
-[[3ql6]] - bPLO + nitro-phenoxymethanesulfonanilide+ SCN + I<br />
-[[3s4f]] - bPLO + pyridine derivative + SCN + I<br />
-[[3r4x]], [[3tuw]] - bPLO + pyrazine derivative + SCN + I<br />
-[[3r5o]] - bPLO + methoxyphenol + SCN + I<br />
-[[3tgy]] - bPLO + ascorbate + SCN + I<br />
-[[3v6q]] - bPLO + CO + SCN + I<br />
-[[2gjm]] - wbPLO + carbonate + SCN + I<br />
-[[3faq]] – wbLPO + CN + SCN + I<br />
-[[3fnl]] - wbPLO + salicylhydroxamic acid + SCN + I<br />
-[[2e9e]] – gPLO + nitrate + carbonate + SCN + CN<br />
-[[2efb]] – gPLO + phosphate + carbonate + SCN + CN<br />
-[[3qf1]] - gPLO + piperazine + SCN + I<br />
-[[2eha]] – gPLO + formate + carbonate + SCN<br />
-[[3r55]] - gPLO + pyrazine derivative + SCN + I<br />
-[[3sxv]] - gPLO + amitrole + SCN + I<br />
-[[3r5q]] - sPLO +tetrahydrofuran + SCN + I<br />