1e15: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1e15.gif|left|200px]]<br /><applet load="1e15" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1e15, resolution 1.90&Aring;" />
-'''CHITINASE B FROM SERRATIA MARCESCENS'''<br />
-==Overview==
+==Chitinase B from Serratia Marcescens==
-In this paper, we describe the structure of chitinase B from Serratia, marcescens, which consists of a catalytic domain with a TIM-barrel fold, and a 49-residue C-terminal chitin-binding domain. This chitinase is the, first structure of a bacterial exochitinase, and it represents one of only, a few examples of a glycosyl hydrolase structure having interacting, catalytic and substrate-binding domains. The chitin-binding domain has, exposed aromatic residues that contribute to a 55-A long continuous, aromatic stretch extending into the active site. Binding of chitin, oligomers is blocked beyond the -3 subsite, which explains why the enzyme, has chitotriosidase activity and degrades the chitin chain from the, nonreducing end. Comparison of the chitinase B structure with that of, chitinase A explains why these enzymes act synergistically in the, degradation of chitin.
+<StructureSection load='1e15' size='340' side='right'caption='[[1e15]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1e15]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E15 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e15 OCA], [https://pdbe.org/1e15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e15 RCSB], [https://www.ebi.ac.uk/pdbsum/1e15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e15 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q54276_SERMA Q54276_SERMA]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/1e15_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e15 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-E15 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E15 OCA].
+*[[Chitinase 3D structures|Chitinase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Structure of a two-domain chitotriosidase from Serratia marcescens at 1.9-A resolution., van Aalten DM, Synstad B, Brurberg MB, Hough E, Riise BW, Eijsink VG, Wierenga RK, Proc Natl Acad Sci U S A. 2000 May 23;97(11):5842-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10823940 10823940]
+[[Category: Large Structures]]
 [[Category: Serratia marcescens]]
-[[Category: Single protein]]
+[[Category: Brurberg MB]]
-[[Category: Aalten, D.M.F.Van.]]
+[[Category: Eijsink VGH]]
-[[Category: Brurberg, M.B.]]
+[[Category: Hough E]]
-[[Category: Eijsink, V.G.H.]]
+[[Category: Riise BW]]
-[[Category: Hough, E.]]
+[[Category: Synstad B]]
-[[Category: Riise, B.W.]]
+[[Category: Van Aalten DMF]]
-[[Category: Synstad, B.]]
+[[Category: Wierenga RK]]
-[[Category: Wierenga, R.K.]]
-[[Category: chitin degradation]]
-[[Category: hydrolase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:55:37 2007''