1e0v: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /> <applet load="1e0v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e0v, resolution 1.7Å" /> '''XYLANASE 10A FROM SR...
 
No edit summary
 
(22 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1e0v.gif|left|200px]]<br />
<applet load="1e0v" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1e0v, resolution 1.7&Aring;" />
'''XYLANASE 10A FROM SREPTOMYCES LIVIDANS. CELLOBIOSYL-ENZYME INTERMEDIATE AT 1.7 A'''<br />


==Overview==
==Xylanase 10A from Sreptomyces lividans. cellobiosyl-enzyme intermediate at 1.7 A==
Endoxylanases are a group of enzymes that hydrolyze the beta-1, 4-linked, xylose backbone of xylans. They are predominantly found in two discrete, sequence families known as glycoside hydrolase families 10 and 11. The, Streptomyces lividans xylanase Xyl10A is a family 10 enzyme, the native, structure of which has previously been determined by x-ray crystallography, at a 2.6 A resolution (Derewenda, U., Swenson, L., Green, R., Wei, Y., Morosoli, R., Shareck, F., Kluepfel, D., and Derewenda, Z. S. (1994) J., Biol. Chem. 269, 20811-20814). Here, we report the native structure of, Xyl10A refined at a resolution of 1.2 A, which reveals many features such, as the rare occurrence of a discretely disordered disulfide bond between, residues Cys-168 and Cys-201. In order to investigate substrate ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10930426 (full description)]]
<StructureSection load='1e0v' size='340' side='right'caption='[[1e0v]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1e0v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E0V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E0V FirstGlance]. <br>
1E0V is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]] with FFC as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E0V OCA]].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=G2F:2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSE'>G2F</scene>, <scene name='pdbligand=PRD_900050:2-deoxy-2-fluoro-beta-cellobiose'>PRD_900050</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e0v OCA], [https://pdbe.org/1e0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e0v RCSB], [https://www.ebi.ac.uk/pdbsum/1e0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e0v ProSAT]</span></td></tr>
Substrate specificity in glycoside hydrolase family 10. Structural and kinetic analysis of the Streptomyces lividans xylanase 10A., Ducros V, Charnock SJ, Derewenda U, Derewenda ZS, Dauter Z, Dupont C, Shareck F, Morosoli R, Kluepfel D, Davies GJ, J Biol Chem. 2000 Jul 28;275(30):23020-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10930426 10930426]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/XYNA_STRLI XYNA_STRLI] Contributes to hydrolyze hemicellulose, the major component of plant cell-walls. XLNA and XLNB seem to act sequentially on the substrate to yield xylobiose and xylose as carbon sources.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e0/1e0v_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e0v ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces lividans]]
[[Category: Streptomyces lividans]]
[[Category: Charnock, S.J.]]
[[Category: Charnock SJ]]
[[Category: Dauter, Z.]]
[[Category: Dauter Z]]
[[Category: Davies, G.J.]]
[[Category: Davies GJ]]
[[Category: Derewenda, U.]]
[[Category: Derewenda U]]
[[Category: Derewenda, Z.S.]]
[[Category: Derewenda ZS]]
[[Category: Ducros, V.]]
[[Category: Ducros V]]
[[Category: Dupont, C.]]
[[Category: Dupont C]]
[[Category: Kluepfel, D.]]
[[Category: Kluepfel D]]
[[Category: Morosoli, R.]]
[[Category: Morosoli R]]
[[Category: Shareck, F.]]
[[Category: Shareck F]]
[[Category: FFC]]
[[Category: glycoside hydrolase family 10]]
[[Category: glycosyl-enzyme intermediate]]
[[Category: xylan degradation]]
[[Category: xylanase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:56:15 2007''

Latest revision as of 12:56, 20 March 2024

Xylanase 10A from Sreptomyces lividans. cellobiosyl-enzyme intermediate at 1.7 AXylanase 10A from Sreptomyces lividans. cellobiosyl-enzyme intermediate at 1.7 A

Structural highlights

1e0v is a 1 chain structure with sequence from Streptomyces lividans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

XYNA_STRLI Contributes to hydrolyze hemicellulose, the major component of plant cell-walls. XLNA and XLNB seem to act sequentially on the substrate to yield xylobiose and xylose as carbon sources.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1e0v, resolution 1.70Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1e0v&oldid=4107678"