1dys: Difference between revisions

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[[Image:1dys.gif|left|200px]]<br />
<applet load="1dys" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1dys, resolution 1.6&Aring;" />
'''ENDOGLUCANASE CEL6B FROM HUMICOLA INSOLENS'''<br />


==Overview==
==Endoglucanase CEL6B from Humicola insolens==
Cellulases are traditionally classified as either endoglucanases or, cellobiohydrolases on the basis of their respective catalytic activities, on crystalline cellulose, which is generally hydrolysed more efficiently, only by the cellobiohydrolases. On the basis of the Trichoderma reesei, cellobiohydrolase II structure, it was proposed that the active-site, tunnel of cellobiohydrolases permitted the processive hydrolysis of, cellulose, whereas the corresponding endoglucanases would display open, active-site clefts [Rouvinen, Bergfors, Teeri, Knowles and Jones (1990), Science 249, 380-386]. Glycoside hydrolase family 6 contains both, cellobiohydrolases and endoglucanases. The structure of the catalytic core, of the family 6 endoglucanase Cel6B from Humicola insolens has been solved, by ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10794732 (full description)]]
<StructureSection load='1dys' size='340' side='right'caption='[[1dys]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1dys]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DYS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dys OCA], [https://pdbe.org/1dys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dys RCSB], [https://www.ebi.ac.uk/pdbsum/1dys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dys ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GUN6_HUMIN GUN6_HUMIN] Plays a central role in the recycling of plant biomass. The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dy/1dys_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dys ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1DYS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]]. Active as [[http://en.wikipedia.org/wiki/Cellulase Cellulase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Structure known Active Sites: CIA and CIB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DYS OCA]].
*[[Glucanase 3D structures|Glucanase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure and function of Humicola insolens family 6 cellulases: structure of the endoglucanase, Cel6B, at 1.6 A resolution., Davies GJ, Brzozowski AM, Dauter M, Varrot A, Schulein M, Biochem J. 2000 May 15;348 Pt 1:201-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10794732 10794732]
[[Category: Cellulase]]
[[Category: Humicola insolens]]
[[Category: Humicola insolens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Brzozowski, A.M.]]
[[Category: Brzozowski AM]]
[[Category: Dauter, M.]]
[[Category: Dauter M]]
[[Category: Davies, G.J.]]
[[Category: Davies GJ]]
[[Category: Schulein, M.]]
[[Category: Schulein M]]
[[Category: Varrot, A.]]
[[Category: Varrot A]]
[[Category: cellulase]]
[[Category: cellulose degradation]]
[[Category: glycoside hydrolase family 6]]
[[Category: hydrolase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:02:44 2007''

Latest revision as of 12:55, 20 March 2024

Endoglucanase CEL6B from Humicola insolensEndoglucanase CEL6B from Humicola insolens

Structural highlights

1dys is a 2 chain structure with sequence from Humicola insolens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GUN6_HUMIN Plays a central role in the recycling of plant biomass. The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1dys, resolution 1.60Å

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