1dvv: Difference between revisions

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OCA

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-[[Image:1dvv.jpg|left|200px]]<br /><applet load="1dvv" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1dvv" />
-'''SOLUTION STRUCTURE OF THE QUINTUPLE MUTANT OF CYTOCHROME C-551 FROM PSEUDOMONAS AERUGINOSA'''<br />
-==Overview==
+==SOLUTION STRUCTURE OF THE QUINTUPLE MUTANT OF CYTOCHROME C-551 FROM PSEUDOMONAS AERUGINOSA==
-Mesophilic cytochrome c(551) of Pseudomonas aeruginosa (PA c(551)) became, as stable as its thermophilic counterpart, Hydrogenobacter thermophilus, cytochrome c(552) (HT c(552)), through only five amino acid substitutions., The five residues, distributed in three spatially separated regions, were, selected and mutated with reference to the corresponding residues in HT, c(552) through careful structure comparison. Thermodynamic analysis, indicated that the stability of the quintuple mutant of PA c(551) could be, partly attained through an enthalpic factor. The solution structure of the, mutant showed that, as in HT c(552), there were tighter side chain, packings in the mutated regions. Furthermore, the mutant had an increased, total accessible surface area, resulting in great negative hydration free, energy. Our results provide a novel example of protein stabilization in, that limited amino acid substitutions can confer the overall stability of, a natural highly thermophilic protein upon a mesophilic molecule.
+<StructureSection load='1dvv' size='340' side='right'caption='[[1dvv]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1dvv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DVV FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dvv OCA], [https://pdbe.org/1dvv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dvv RCSB], [https://www.ebi.ac.uk/pdbsum/1dvv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dvv ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CY551_PSEAE CY551_PSEAE] Electron donor for cytochrome cd1 in nitrite and nitrate respiration.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dv/1dvv_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dvv ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-DVV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DVV OCA].
+*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Selected mutations in a mesophilic cytochrome c confer the stability of a thermophilic counterpart., Hasegawa J, Uchiyama S, Tanimoto Y, Mizutani M, Kobayashi Y, Sambongi Y, Igarashi Y, J Biol Chem. 2000 Dec 1;275(48):37824-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10918067 10918067]
+[[Category: Large Structures]]
 [[Category: Pseudomonas aeruginosa]]
-[[Category: Single protein]]
+[[Category: Hasegawa J]]
-[[Category: Hasegawa, J.]]
+[[Category: Igarashi Y]]
-[[Category: Igarashi, Y.]]
+[[Category: Kobayashi Y]]
-[[Category: Kobayashi, Y.]]
+[[Category: Mizutani M]]
-[[Category: Mizutani, M.]]
+[[Category: Sambongi Y]]
-[[Category: Sambongi, Y.]]
+[[Category: Tanimoto Y]]
-[[Category: Tanimoto, Y.]]
+[[Category: Uchiyama S]]
-[[Category: Uchiyama, S.]]
-[[Category: HEM]]
-[[Category: cytochrome c]]
-[[Category: stability]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:37:46 2007''