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[[Image:1dvv.jpg|left|200px]]


{{Structure
==SOLUTION STRUCTURE OF THE QUINTUPLE MUTANT OF CYTOCHROME C-551 FROM PSEUDOMONAS AERUGINOSA==
|PDB= 1dvv |SIZE=350|CAPTION= <scene name='initialview01'>1dvv</scene>
<StructureSection load='1dvv' size='340' side='right'caption='[[1dvv]]' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>
<table><tr><td colspan='2'>[[1dvv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DVV FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dvv OCA], [https://pdbe.org/1dvv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dvv RCSB], [https://www.ebi.ac.uk/pdbsum/1dvv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dvv ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dvv OCA], [http://www.ebi.ac.uk/pdbsum/1dvv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dvv RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/CY551_PSEAE CY551_PSEAE] Electron donor for cytochrome cd1 in nitrite and nitrate respiration.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dv/1dvv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dvv ConSurf].
<div style="clear:both"></div>


'''SOLUTION STRUCTURE OF THE QUINTUPLE MUTANT OF CYTOCHROME C-551 FROM PSEUDOMONAS AERUGINOSA'''
==See Also==
 
*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Mesophilic cytochrome c(551) of Pseudomonas aeruginosa (PA c(551)) became as stable as its thermophilic counterpart, Hydrogenobacter thermophilus cytochrome c(552) (HT c(552)), through only five amino acid substitutions. The five residues, distributed in three spatially separated regions, were selected and mutated with reference to the corresponding residues in HT c(552) through careful structure comparison. Thermodynamic analysis indicated that the stability of the quintuple mutant of PA c(551) could be partly attained through an enthalpic factor. The solution structure of the mutant showed that, as in HT c(552), there were tighter side chain packings in the mutated regions. Furthermore, the mutant had an increased total accessible surface area, resulting in great negative hydration free energy. Our results provide a novel example of protein stabilization in that limited amino acid substitutions can confer the overall stability of a natural highly thermophilic protein upon a mesophilic molecule.
[[Category: Large Structures]]
 
==About this Structure==
1DVV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DVV OCA].
 
==Reference==
Selected mutations in a mesophilic cytochrome c confer the stability of a thermophilic counterpart., Hasegawa J, Uchiyama S, Tanimoto Y, Mizutani M, Kobayashi Y, Sambongi Y, Igarashi Y, J Biol Chem. 2000 Dec 1;275(48):37824-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10918067 10918067]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Single protein]]
[[Category: Hasegawa J]]
[[Category: Hasegawa, J.]]
[[Category: Igarashi Y]]
[[Category: Igarashi, Y.]]
[[Category: Kobayashi Y]]
[[Category: Kobayashi, Y.]]
[[Category: Mizutani M]]
[[Category: Mizutani, M.]]
[[Category: Sambongi Y]]
[[Category: Sambongi, Y.]]
[[Category: Tanimoto Y]]
[[Category: Tanimoto, Y.]]
[[Category: Uchiyama S]]
[[Category: Uchiyama, S.]]
[[Category: cytochrome c]]
[[Category: stability]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:49:49 2008''

Latest revision as of 12:54, 20 March 2024

SOLUTION STRUCTURE OF THE QUINTUPLE MUTANT OF CYTOCHROME C-551 FROM PSEUDOMONAS AERUGINOSASOLUTION STRUCTURE OF THE QUINTUPLE MUTANT OF CYTOCHROME C-551 FROM PSEUDOMONAS AERUGINOSA

Structural highlights

1dvv is a 1 chain structure with sequence from Pseudomonas aeruginosa. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CY551_PSEAE Electron donor for cytochrome cd1 in nitrite and nitrate respiration.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

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