1dt7: Difference between revisions

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-[[Image:1dt7.gif|left|200px]]<br /><applet load="1dt7" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1dt7" />
-'''SOLUTION STRUCTURE OF THE C-TERMINAL NEGATIVE REGULATORY DOMAIN OF P53 IN A COMPLEX WITH CA2+-BOUND S100B(BB)'''<br />
-==Overview==
+==SOLUTION STRUCTURE OF THE C-TERMINAL NEGATIVE REGULATORY DOMAIN OF P53 IN A COMPLEX WITH CA2+-BOUND S100B(BB)==
-A Ca2+ dependent conformational change in dimeric S100B(betabeta) is, required for it to bind p53 and inhibit phosphorylation of this tumor, suppressor in its C-terminal negative regulatory domain. A peptide derived, from this region of p53 (residues 367-388) was found to have no regular, structure in its native form by NMR spectroscopy, but becomes helical when, bound to Ca2+ loaded S100B(betabeta). The three-dimensional structure of, this complex reveals several favorable hydrophobic and electrostatic, interactions between S100B(betabeta) and the p53 peptide in the binding, pocket, where S100B(betabeta) sterically blocks sites of phosphorylation, and acetylation on p53 that are important for transcription activation.
+<StructureSection load='1dt7' size='340' side='right'caption='[[1dt7]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1dt7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DT7 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dt7 OCA], [https://pdbe.org/1dt7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dt7 RCSB], [https://www.ebi.ac.uk/pdbsum/1dt7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dt7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/S100B_RAT S100B_RAT] Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization.<ref>PMID:19910580</ref> <ref>PMID:20351179</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dt/1dt7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dt7 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-DT7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DT7 OCA].
+*[[S100 proteins 3D structures|S100 proteins 3D structures]]
+== References ==
-==Reference==
+<references/>
-Structure of the negative regulatory domain of p53 bound to S100B(betabeta)., Rustandi RR, Baldisseri DM, Weber DJ, Nat Struct Biol. 2000 Jul;7(7):570-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10876243 10876243]
+__TOC__
-[[Category: Protein complex]]
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
 [[Category: Rattus norvegicus]]
-[[Category: Baldisseri, D.M.]]
+[[Category: Baldisseri DM]]
-[[Category: Rustandi, R.R.]]
+[[Category: Rustandi RR]]
-[[Category: Weber, D.J.]]
+[[Category: Weber DJ]]
-[[Category: CA]]
-[[Category: c-terminal domain of p53]]
-[[Category: calcium-binding]]
-[[Category: ef-hand]]
-[[Category: four helix bundle]]
-[[Category: helix loop helix]]
-[[Category: nmr]]
-[[Category: p53]]
-[[Category: s100 protein]]
-[[Category: s100b]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:42:03 2008''