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==SAM-bound SAM-IV riboswitch==
==SAM-bound SAM-IV riboswitch==
<StructureSection load='6uet' size='340' side='right'caption='[[6uet]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
<SX load='6uet' size='340' side='right' viewer='molstar' caption='[[6uet]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6uet]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UET FirstGlance]. <br>
<table><tr><td colspan='2'>[[6uet]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_sp._MCS Mycobacterium sp. MCS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UET FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uet OCA], [http://pdbe.org/6uet PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uet RCSB], [http://www.ebi.ac.uk/pdbsum/6uet PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uet ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uet OCA], [https://pdbe.org/6uet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uet RCSB], [https://www.ebi.ac.uk/pdbsum/6uet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uet ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Specimens below 50 kDa have generally been considered too small to be analyzed by single-particle cryo-electron microscopy (cryo-EM). The high flexibility of pure RNAs makes it difficult to obtain high-resolution structures by cryo-EM. In bacteria, riboswitches regulate sulfur metabolism through binding to the S-adenosylmethionine (SAM) ligand and offer compelling targets for new antibiotics. SAM-I, SAM-I/IV, and SAM-IV are the three most commonly found SAM riboswitches, but the structure of SAM-IV is still unknown. Here, we report the structures of apo and SAM-bound SAM-IV riboswitches (119-nt, ~40 kDa) to 3.7 A and 4.1 A resolution, respectively, using cryo-EM. The structures illustrate homologies in the ligand-binding core but distinct peripheral tertiary contacts in SAM-IV compared to SAM-I and SAM-I/IV. Our results demonstrate the feasibility of resolving small RNAs with enough detail to enable detection of their ligand-binding pockets and suggest that cryo-EM could play a role in structure-assisted drug design for RNA.


Cryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 A resolution.,Zhang K, Li S, Kappel K, Pintilie G, Su Z, Mou TC, Schmid MF, Das R, Chiu W Nat Commun. 2019 Dec 3;10(1):5511. doi: 10.1038/s41467-019-13494-7. PMID:31796736<ref>PMID:31796736</ref>
==See Also==
 
*[[Riboswitch 3D structures|Riboswitch 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6uet" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chiu, W]]
[[Category: Mycobacterium sp. MCS]]
[[Category: Das, R]]
[[Category: Chiu W]]
[[Category: Kappel, K]]
[[Category: Das R]]
[[Category: Li, S]]
[[Category: Kappel K]]
[[Category: Mou, T]]
[[Category: Li S]]
[[Category: Pintilie, G]]
[[Category: Mou T]]
[[Category: Schmid, M]]
[[Category: Pintilie G]]
[[Category: Su, Z]]
[[Category: Schmid M]]
[[Category: Zhang, K]]
[[Category: Su Z]]
[[Category: Cryo-em]]
[[Category: Zhang K]]
[[Category: Rna]]
[[Category: Sam-iv riboswitch]]
[[Category: Small rna]]

Latest revision as of 12:29, 20 March 2024

SAM-bound SAM-IV riboswitchSAM-bound SAM-IV riboswitch

6uet, resolution 4.10Å

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