6u5f: Difference between revisions

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<StructureSection load='6u5f' size='340' side='right'caption='[[6u5f]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
<StructureSection load='6u5f' size='340' side='right'caption='[[6u5f]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6u5f]] is a 54 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U5F OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U5F FirstGlance]. <br>
<table><tr><td colspan='2'>[[6u5f]] is a 54 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U5F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U5F FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6pyt|6pyt]], [[6u5b|6u5b]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u5f OCA], [http://pdbe.org/6u5f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u5f RCSB], [http://www.ebi.ac.uk/pdbsum/6u5f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u5f ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u5f OCA], [https://pdbe.org/6u5f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u5f RCSB], [https://www.ebi.ac.uk/pdbsum/6u5f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u5f ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/G3XD82_PSEAE G3XD82_PSEAE]
R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics(1-4). Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded in a metastable state by a baseplate scaffold(1,2). Fine-tuning of such nucleic acid-free protein machines for precision medicine calls for an atomic description of the entire complex and contraction mechanism, which is not available from baseplate structures of the (DNA-containing) T4 bacteriophage(5). Here we report the atomic model of the complete R2 pyocin in its pre-contraction and post-contraction states, each containing 384 subunits of 11 unique atomic models of 10 gene products. Comparison of these structures suggests the following sequence of events during pyocin contraction: tail fibres trigger lateral dissociation of baseplate triplexes; the dissociation then initiates a cascade of events leading to sheath contraction; and this contraction converts chemical energy into mechanical force to drive the iron-tipped tube across the bacterial cell surface, killing the bacterium.
 
Action of a minimal contractile bactericidal nanomachine.,Ge P, Scholl D, Prokhorov NS, Avaylon J, Shneider MM, Browning C, Buth SA, Plattner M, Chakraborty U, Ding K, Leiman PG, Miller JF, Zhou ZH Nature. 2020 Apr;580(7805):658-662. doi: 10.1038/s41586-020-2186-z. Epub 2020 Apr, 15. PMID:32350467<ref>PMID:32350467</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6u5f" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Pseae]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Avaylon, J]]
[[Category: Avaylon J]]
[[Category: Browning, C]]
[[Category: Browning C]]
[[Category: Buth, S A]]
[[Category: Buth SA]]
[[Category: Ding, K]]
[[Category: Ding K]]
[[Category: Ge, P]]
[[Category: Ge P]]
[[Category: Leiman, P G]]
[[Category: Leiman PG]]
[[Category: Miller, J F]]
[[Category: Miller JF]]
[[Category: Plattner, M]]
[[Category: Plattner M]]
[[Category: Scholl, D]]
[[Category: Scholl D]]
[[Category: Shneider, M M]]
[[Category: Shneider MM]]
[[Category: Zhou, Z H]]
[[Category: Zhou ZH]]
[[Category: Antimicrobial protein]]
[[Category: Bacteriocin]]
[[Category: Pyocin]]

Latest revision as of 12:28, 20 March 2024

CryoEM Structure of Pyocin R2 - precontracted - collarCryoEM Structure of Pyocin R2 - precontracted - collar

Structural highlights

6u5f is a 54 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

G3XD82_PSEAE

6u5f, resolution 3.80Å

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