|
|
| (One intermediate revision by the same user not shown) |
| Line 3: |
Line 3: |
| <SX load='6pom' size='340' side='right' viewer='molstar' caption='[[6pom]], [[Resolution|resolution]] 4.90Å' scene=''> | | <SX load='6pom' size='340' side='right' viewer='molstar' caption='[[6pom]], [[Resolution|resolution]] 4.90Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6pom]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6POM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6POM FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6pom]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6POM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6POM FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6pmo|6pmo]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.9Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pom OCA], [http://pdbe.org/6pom PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pom RCSB], [http://www.ebi.ac.uk/pdbsum/6pom PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pom ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pom OCA], [https://pdbe.org/6pom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pom RCSB], [https://www.ebi.ac.uk/pdbsum/6pom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pom ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| Amino acid availability in Gram-positive bacteria is monitored by T-box riboswitches. T-boxes directly bind tRNAs, assess their aminoacylation state, and regulate the transcription or translation of downstream genes to maintain nutritional homeostasis. Here, we report cocrystal and cryo-EM structures of Geobacillus kaustophilus and Bacillus subtilis T-box-tRNA complexes, detailing their multivalent, exquisitely selective interactions. The T-box forms a U-shaped molecular vise that clamps the tRNA, captures its 3' end using an elaborate 'discriminator' structure, and interrogates its aminoacylation state using a steric filter fashioned from a wobble base pair. In the absence of aminoacylation, T-boxes clutch tRNAs and form a continuously stacked central spine, permitting transcriptional readthrough or translation initiation. A modeled aminoacyl disrupts tRNA-T-box stacking, severing the central spine and blocking gene expression. Our data establish a universal mechanism of amino acid sensing on tRNAs and gene regulation by T-box riboswitches and exemplify how higher-order RNA-RNA interactions achieve multivalency and specificity.
| |
|
| |
|
| Structural basis of amino acid surveillance by higher-order tRNA-mRNA interactions.,Li S, Su Z, Lehmann J, Stamatopoulou V, Giarimoglou N, Henderson FE, Fan L, Pintilie GD, Zhang K, Chen M, Ludtke SJ, Wang YX, Stathopoulos C, Chiu W, Zhang J Nat Struct Mol Biol. 2019 Nov 18. pii: 10.1038/s41594-019-0326-7. doi:, 10.1038/s41594-019-0326-7. PMID:31740854<ref>PMID:31740854</ref>
| | ==See Also== |
| | | *[[Riboswitch 3D structures|Riboswitch 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 6pom" style="background-color:#fffaf0;"></div>
| |
| == References == | |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| | [[Category: Bacillus subtilis]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Chiu, W]] | | [[Category: Chiu W]] |
| [[Category: Li, S]] | | [[Category: Li S]] |
| [[Category: Su, Z]] | | [[Category: Su Z]] |
| [[Category: Zhang, J]] | | [[Category: Zhang J]] |
| [[Category: Riboswitch]]
| |
| [[Category: Rna]]
| |
| [[Category: Rna complex]]
| |
| [[Category: Stacking]]
| |
| [[Category: Transcription attenuation]]
| |