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| ==Cryo-EM structure of the C2-symmetric TRPV2/RTx complex in nanodiscs== | | ==Cryo-EM structure of the C2-symmetric TRPV2/RTx complex in nanodiscs== |
| <StructureSection load='6oo7' size='340' side='right'caption='[[6oo7]], [[Resolution|resolution]] 3.80Å' scene=''> | | <SX load='6oo7' size='340' side='right' viewer='molstar' caption='[[6oo7]], [[Resolution|resolution]] 3.80Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6oo7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OO7 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6oo7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OO7 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6EU:RESINIFERATOXIN'>6EU</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TRPV2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6EU:RESINIFERATOXIN'>6EU</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oo7 OCA], [http://pdbe.org/6oo7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oo7 RCSB], [http://www.ebi.ac.uk/pdbsum/6oo7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oo7 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oo7 OCA], [https://pdbe.org/6oo7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oo7 RCSB], [https://www.ebi.ac.uk/pdbsum/6oo7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oo7 ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/G1SNM3_RABIT G1SNM3_RABIT] |
| The Transient Receptor Potential Vanilloid 2 (TRPV2) channel is a member of the temperature-sensing thermoTRPV family. Recent advances in cryo-electronmicroscopy (cryo-EM) and X-ray crystallography have provided many important insights into the gating mechanisms of thermoTRPV channels. Interestingly, crystallographic studies of ligand-dependent TRPV2 gating have shown that the TRPV2 channel adopts two-fold symmetric arrangements during the gating cycle. However, it was unclear if crystal packing forces played a role in stabilizing the two-fold symmetric arrangement of the channel. Here we employ cryo-EM to elucidate the structure of full-length rabbit TRPV2 in complex with the agonist resiniferatoxin (RTx) in nanodiscs and amphipol. We show that RTx induces two-fold symmetric conformations of TRPV2 in both environments. However, the two-fold symmetry is more pronounced in the native-like lipid environment of the nanodiscs. Our data offers insights into a gating pathway in TRPV2 involving symmetry transitions.
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| Symmetry transitions during gating of the TRPV2 ion channel in lipid membranes.,Zubcevic L, Hsu AL, Borgnia MJ, Lee SY Elife. 2019 May 15;8. pii: 45779. doi: 10.7554/eLife.45779. PMID:31090543<ref>PMID:31090543</ref>
| | ==See Also== |
| | | *[[Ion channels 3D structures|Ion channels 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6oo7" style="background-color:#fffaf0;"></div>
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| == References == | |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </SX> |
| [[Category: European rabbit]]
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| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Borgnia, M J]] | | [[Category: Oryctolagus cuniculus]] |
| [[Category: Hsu, A L]] | | [[Category: Borgnia MJ]] |
| [[Category: Lee, S Y]] | | [[Category: Hsu AL]] |
| [[Category: Zubcevic, L]] | | [[Category: Lee S-Y]] |
| [[Category: Calcium channel]]
| | [[Category: Zubcevic L]] |
| [[Category: Ion channel]]
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| [[Category: Metal transport]]
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| [[Category: Trp channel]]
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