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==Single particle reconstruction of the symmetric core an engineered protein scaffold==
==Single particle reconstruction of the symmetric core an engineered protein scaffold==
<StructureSection load='6nht' size='340' side='right'caption='[[6nht]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
<SX load='6nht' size='340' side='right' viewer='molstar' caption='[[6nht]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6nht]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae] and [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NHT FirstGlance]. <br>
<table><tr><td colspan='2'>[[6nht]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1] and [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NHT FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA1966 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nht OCA], [http://pdbe.org/6nht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nht RCSB], [http://www.ebi.ac.uk/pdbsum/6nht PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nht ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nht OCA], [https://pdbe.org/6nht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nht RCSB], [https://www.ebi.ac.uk/pdbsum/6nht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nht ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q9I2D8_PSEAE Q9I2D8_PSEAE]
Proteins smaller than about 50 kDa are currently too small to be imaged at high resolution by cryo-electron microscopy (cryo-EM), leaving most protein molecules in the cell beyond the reach of this powerful structural technique. Here we use a designed protein scaffold to bind and symmetrically display 12 copies of a small 26 kDa protein, green fluorescent protein (GFP). We show that the bound cargo protein is held rigidly enough to visualize it at a resolution of 3.8 A by cryo-EM, where specific structural features of the protein are visible. The designed scaffold is modular and can be modified through modest changes in its amino acid sequence to bind and display diverse proteins for imaging, thus providing a general method to break through the lower size limitation in cryo-EM.
 
A 3.8 A resolution cryo-EM structure of a small protein bound to an imaging scaffold.,Liu Y, Huynh DT, Yeates TO Nat Commun. 2019 Apr 23;10(1):1864. doi: 10.1038/s41467-019-09836-0. PMID:31015551<ref>PMID:31015551</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6nht" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Pseae]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Pyrococcus horikoshii]]
[[Category: Pyrococcus horikoshii OT3]]
[[Category: Huynh, D]]
[[Category: Huynh D]]
[[Category: Liu, Y]]
[[Category: Liu Y]]
[[Category: Yeates, T O]]
[[Category: Yeates TO]]
[[Category: Biosynthetic protein]]
[[Category: Display platform]]
[[Category: Protein engineering]]
[[Category: Small protein cryo-em]]
[[Category: Symmetric scaffold]]

Latest revision as of 12:21, 20 March 2024

Single particle reconstruction of the symmetric core an engineered protein scaffoldSingle particle reconstruction of the symmetric core an engineered protein scaffold

6nht, resolution 2.90Å

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