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<StructureSection load='4ysq' size='340' side='right'caption='[[4ysq]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='4ysq' size='340' side='right'caption='[[4ysq]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4ysq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geotn Geotn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YSQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YSQ FirstGlance]. <br>
<table><tr><td colspan='2'>[[4ysq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_thermodenitrificans_NG80-2 Geobacillus thermodenitrificans NG80-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YSQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YSQ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ysa|4ysa]], [[4ysc|4ysc]], [[4ysd|4ysd]], [[4yse|4yse]], [[4yso|4yso]], [[4ysp|4ysp]], [[4yss|4yss]], [[4yst|4yst]], [[4ysu|4ysu]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nirK, GTNG_0650 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=420246 GEOTN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ysq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ysq OCA], [https://pdbe.org/4ysq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ysq RCSB], [https://www.ebi.ac.uk/pdbsum/4ysq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ysq ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ysq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ysq OCA], [http://pdbe.org/4ysq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ysq RCSB], [http://www.ebi.ac.uk/pdbsum/4ysq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ysq ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A4IL26_GEOTN A4IL26_GEOTN]
Serial femtosecond crystallography (SFX) has enabled the damage-free structural determination of metalloenzymes and filled the gaps of our knowledge between crystallographic and spectroscopic data. Crystallographers, however, scarcely know whether the rising technique provides truly new structural insights into mechanisms of metalloenzymes partly because of limited resolutions. Copper nitrite reductase (CuNiR), which converts nitrite to nitric oxide in denitrification, has been extensively studied by synchrotron radiation crystallography (SRX). Although catalytic Cu (Type 2 copper (T2Cu)) of CuNiR had been suspected to tolerate X-ray photoreduction, we here showed that T2Cu in the form free of nitrite is reduced and changes its coordination structure in SRX. Moreover, we determined the completely oxidized CuNiR structure at 1.43 A resolution with SFX. Comparison between the high-resolution SFX and SRX data revealed the subtle structural change of a catalytic His residue by X-ray photoreduction. This finding, which SRX has failed to uncover, provides new insight into the reaction mechanism of CuNiR.
 
Redox-coupled structural changes in nitrite reductase revealed by serial femtosecond and microfocus crystallography.,Fukuda Y, Tse KM, Suzuki M, Diederichs K, Hirata K, Nakane T, Sugahara M, Nango E, Tono K, Joti Y, Kameshima T, Song C, Hatsui T, Yabashi M, Nureki O, Matsumura H, Inoue T, Iwata S, Mizohata E J Biochem. 2016 Jan 14. pii: mvv133. PMID:26769972<ref>PMID:26769972</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ysq" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Nitrite reductase 3D structures|Nitrite reductase 3D structures]]
*[[Nitrite reductase 3D structures|Nitrite reductase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Geotn]]
[[Category: Geobacillus thermodenitrificans NG80-2]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Diederichs, K]]
[[Category: Diederichs K]]
[[Category: Fukuda, Y]]
[[Category: Fukuda Y]]
[[Category: Hatsui, T]]
[[Category: Hatsui T]]
[[Category: Hirata, K]]
[[Category: Hirata K]]
[[Category: Inoue, T]]
[[Category: Inoue T]]
[[Category: Iwata, S]]
[[Category: Iwata S]]
[[Category: Joti, Y]]
[[Category: Joti Y]]
[[Category: Kameshima, T]]
[[Category: Kameshima T]]
[[Category: Matsumura, H]]
[[Category: Matsumura H]]
[[Category: Mizohata, E]]
[[Category: Mizohata E]]
[[Category: Nakane, T]]
[[Category: Nakane T]]
[[Category: Nango, E]]
[[Category: Nango E]]
[[Category: Nureki, O]]
[[Category: Nureki O]]
[[Category: Song, C]]
[[Category: Song C]]
[[Category: Sugahara, M]]
[[Category: Sugahara M]]
[[Category: Suzuki, M]]
[[Category: Suzuki M]]
[[Category: Tono, K]]
[[Category: Tono K]]
[[Category: Tse, K M]]
[[Category: Tse KM]]
[[Category: Yabashi, M]]
[[Category: Yabashi M]]
[[Category: Copper]]
[[Category: Nitrite]]
[[Category: Oxidoreductase]]
[[Category: Reductase]]

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