4xd9: Difference between revisions

<StructureSection load='4xd9' size='340' side='right' caption='[[4xd9]], [[Resolution|resolution]] 2.35&Aring;' scene=''>

<table><tr><td colspan='2'>[[4xd9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XD9 FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xd9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xd9 RCSB], [http://www.ebi.ac.uk/pdbsum/4xd9 PDBsum]</span></td></tr>

== Publication Abstract from PubMed ==

Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. These proteins are necessary for the recruitment of three ribosomal components (5S ribosomal RNA [rRNA], RpL5 and RpL11) to the 90S ribosome precursor and subsequent 27SB pre-rRNA processing. Here we present the crystal structure of the Aspergillus nidulans (An) Rpf2-Rrs1 core complex. The core complex contains the tightly interlocked N-terminal domains of Rpf2 and Rrs1. The Rpf2 N-terminal domain includes a Brix domain characterized by similar N- and C-terminal architecture. The long alpha-helix of Rrs1 joins the C-terminal half of the Brix domain as if it were part of a single molecule. The conserved proline-rich linker connecting the N- and C-terminal domains of Rrs1 wrap around the side of Rpf2 and anchor the C-terminal domain of Rrs1 to a specific site on Rpf2. In addition, gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA. Further analysis of Rpf2-Rrs1 mutants demonstrated that Saccharomyces cerevisiae Rpf2 R236 (corresponds to R238 of AnRpf2) plays a significant role in this binding. Based on these studies and previous reports, we have proposed a model for ribosomal component recruitment to the 90S ribosome precursor.

 

Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis.,Asano N, Kato K, Nakamura A, Komoda K, Tanaka I, Yao M Nucleic Acids Res. 2015 May 19;43(9):4746-57. doi: 10.1093/nar/gkv305. Epub 2015 , Apr 8. PMID:25855814<ref>PMID:25855814</ref>

== References ==

[[Category: Asano, N]]

[[Category: Kato, K]]

[[Category: Yao, M]]

[[Category: Complex]]

[[Category: Translation]]