4nnh: Difference between revisions
New page: '''Unreleased structure''' The entry 4nnh is ON HOLD Authors: Juanjuan Yang, J.Y., Yindi Liu, Y.L., Qixu cai, Q.C., Donghai Lin, D.L., the nuclear magnetic resonance research center De... |
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The | ==Structural basis for targeting the ribosomal protein S1 of Mycobacterium tuberculosis by pyrazinamide== | ||
<StructureSection load='4nnh' size='340' side='right'caption='[[4nnh]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4nnh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NNH FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nnh OCA], [https://pdbe.org/4nnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nnh RCSB], [https://www.ebi.ac.uk/pdbsum/4nnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nnh ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RS1_MYCS2 RS1_MYCS2] Binds mRNA, facilitating recognition of most mRNAs by the 30S ribosomal subunit during translation initiation (By similarity). Plays a role in trans-translation; binds tmRNA (the product of the ssrA gene) (PubMed:25430994). Binds very poorly to pyrazinoic acid (POA), the active form of the prodrug pyrazinamide (PZA); POA does not disrupt trans-translation in this organism (PubMed:21835980). M.smegmatis is resistant to the antibiotic PZA (PubMed:25430994). In trans-translation Ala-aminoacylated transfer-messenger RNA (tmRNA, product of the ssrA gene; the 2 termini fold to resemble tRNA(Ala) while it encodes a short internal open reading frame (the tag peptide)) acts like a tRNA, entering the A-site of the ribosome and displacing the stalled mRNA (which is subsequently degraded). The ribosome then switches to translate the ORF on the tmRNA, the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and thus targeted for degradation.[UniProtKB:P0AG67]<ref>PMID:21835980</ref> <ref>PMID:25430994</ref> | |||
==See Also== | |||
*[[Ribosomal protein S1|Ribosomal protein S1]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mycolicibacterium smegmatis MC2 155]] | |||
[[Category: Cai Q]] | |||
[[Category: Lin D]] | |||
[[Category: Liu Y]] | |||
[[Category: Yang J]] | |||