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==Crystal structure of Mycobacterium tuberculosis UgpB==
==Crystal structure of Mycobacterium tuberculosis UgpB==
<StructureSection load='4mfi' size='340' side='right' caption='[[4mfi]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='4mfi' size='340' side='right'caption='[[4mfi]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4mfi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MFI FirstGlance]. <br>
<table><tr><td colspan='2'>[[4mfi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Ra Mycobacterium tuberculosis H37Ra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MFI FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mfi RCSB], [http://www.ebi.ac.uk/pdbsum/4mfi PDBsum]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<table>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mfi OCA], [https://pdbe.org/4mfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mfi RCSB], [https://www.ebi.ac.uk/pdbsum/4mfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mfi ProSAT]</span></td></tr>
<div style="background-color:#fffaf0;">
</table>
== Publication Abstract from PubMed ==
== Function ==
Tuberculosis (TB), caused by Mycobacterium tuberculosis, is one of the most devastating human diseases, and is responsible for ~ 2 million deaths worldwide each year. The nutritional requirements for the growth of mycobacteria have been extensively studied since the discovery of M. tuberculosis, but the essential nutrients for M. tuberculosis inside the human host and the identity of the corresponding transporters remain unknown. The UgpABCE transporter of M. tuberculosis is one of five putative permeases for carbohydrate uptake, and is genetically predicted to be an sn-glycerol 3-phosphate importer. We have determined the 1.5-A crystal structure of M. tuberculosis UgpB, which has been reported to be a promising vaccine candidate against TB. M. tuberculosis UgpB showed no detectable binding activity for sn-glycerol 3-phosphate by isothermal titration calorimetry, but instead showed a preference for glycerophosphocholine (GPC). M. tuberculosis UgpB largely resembles its Escherichia coli homolog, but with the critical Trp169 in the substrate-binding site of E. coli UgpB replaced by Leu205. Mutation of Leu205 abolishes GPC binding, suggesting that Leu205 is a determinant of GPC binding. The work reported here not only contributes to our understanding of the carbon and phosphate sources utilized by M. tuberculosis inside the human host, but will also promote improvements in TB chemotherapy. Database: Structural data are available in the Protein Data Bank database under the accession number PDB 4MFI.
[https://www.uniprot.org/uniprot/A5U6I5_MYCTA A5U6I5_MYCTA]
 
Structural analysis of Mycobacterium tuberculosis ATP-binding cassette transporter subunit UgpB reveals specificity for glycerophosphocholine.,Jiang D, Zhang Q, Zheng Q, Zhou H, Jin J, Zhou W, Bartlam M, Rao Z FEBS J. 2014 Jan;281(1):331-41. doi: 10.1111/febs.12600. Epub 2013 Dec 2. PMID:24299297<ref>PMID:24299297</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
== References ==
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bartlam, M.]]
[[Category: Large Structures]]
[[Category: Jiang, D.]]
[[Category: Mycobacterium tuberculosis H37Ra]]
[[Category: Rao, Z.]]
[[Category: Bartlam M]]
[[Category: Abc transporter substrate-binding protein]]
[[Category: Jiang D]]
[[Category: Alpha/beta domain]]
[[Category: Rao Z]]
[[Category: Carbohydrate/sugar binding]]
[[Category: Sugar binding protein]]

Latest revision as of 11:56, 20 March 2024

Crystal structure of Mycobacterium tuberculosis UgpBCrystal structure of Mycobacterium tuberculosis UgpB

Structural highlights

4mfi is a 1 chain structure with sequence from Mycobacterium tuberculosis H37Ra. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.5Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A5U6I5_MYCTA

See Also

4mfi, resolution 1.50Å

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