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| ==Crystal structure of a Stt7 homolog from Micromonas algae== | | ==Crystal structure of a Stt7 homolog from Micromonas algae== |
| <StructureSection load='4ix3' size='340' side='right' caption='[[4ix3]], [[Resolution|resolution]] 1.35Å' scene=''> | | <StructureSection load='4ix3' size='340' side='right'caption='[[4ix3]], [[Resolution|resolution]] 1.35Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4ix3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Micromonas_sp._rcc299 Micromonas sp. rcc299]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IX3 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4ix3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonas_commoda Micromonas commoda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IX3 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ix4|4ix4]], [[4ix5|4ix5]], [[4ix6|4ix6]]</td></tr>
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">STT7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=296587 Micromonas sp. RCC299])</td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ix3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ix3 OCA], [https://pdbe.org/4ix3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ix3 RCSB], [https://www.ebi.ac.uk/pdbsum/4ix3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ix3 ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ix3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ix3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ix3 RCSB], [http://www.ebi.ac.uk/pdbsum/4ix3 PDBsum]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/C1EBN1_MICCC C1EBN1_MICCC] |
| Under natural environments, plants and algae have evolved various photosynthetic acclimation mechanisms in response to the constantly changing light conditions. The state transition and long-term response processes in photosynthetic acclimation involve remodeling and composition alteration of thylakoid membrane. A chloroplast protein kinase named Stt7/STN7 has been found to have pivotal roles in both state transition and long-term response. Here we report the crystal structures of the kinase domain of a putative Stt7/STN7 homolog from Micromonas sp. RCC299 (MsStt7d) in the apo form and in complex with various nucleotide substrates. MsStt7d adopts a canonical protein kinase fold and contains all the essential residues at the active site. A novel hairpin motif, found to be a conserved feature of the Stt7/STN7 family and indispensable for the kinase stability, interacts with the activation loop and fixes it in an active conformation. We have also demonstrated that MsStt7d is a dualspecifi city kinase that phosphorylates both Thr and Tyr residues. Moreover, preliminary in vitro data suggest that it might be capable of phosphorylating a consensus N-terminal pentapeptide of light-harvesting proteins Micromonas Lhcp4 and Arabidopsis Lhcb1 directly. The potential peptide/protein substrate binding site is predicted based on the location of a pseudo-substrate contributed by the adjacent molecule within the crystallographic dimer. The structural and biochemical data presented here provide a framework for an improved understanding on the role of Stt7/STN7 in photosynthetic acclimation.
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| Structure of the catalytic domain of a state transition kinase homolog from Micromonas algae.,Guo J, Wei X, Li M, Pan X, Chang W, Liu Z Protein Cell. 2013 Aug;4(8):607-19. doi: 10.1007/s13238-013-3034-9. Epub 2013 Jun, 23. PMID:23794031<ref>PMID:23794031</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Micromonas sp. rcc299]] | | [[Category: Large Structures]] |
| [[Category: Chang, W]] | | [[Category: Micromonas commoda]] |
| [[Category: Guo, J]] | | [[Category: Chang W]] |
| [[Category: Li, M]] | | [[Category: Guo J]] |
| [[Category: Liu, Z]] | | [[Category: Li M]] |
| [[Category: Pan, X]] | | [[Category: Liu Z]] |
| [[Category: Wei, X]] | | [[Category: Pan X]] |
| [[Category: Atp binding]]
| | [[Category: Wei X]] |
| [[Category: Canonical protein kinase fold]]
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| [[Category: Protein kinase]]
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| [[Category: Transferase]]
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